We narrowed to 1,978 results for: ENDOG

1. LLP782_Traptavidin-KRAB-CO

   Plasmid

   #211775

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-DNMT3A
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP785_Traptavidin-DNMT3A-CO

   Plasmid

   #211778

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-EZH2
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP781_SnoopCatcher-KRAB-CO

   Plasmid

   #211774

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-KRAB
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNHT7::UMSBP::sNluc2_ORF1

   Plasmid

   #186765

   Purpose

   An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   Nanoluciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. GSX2-P2A-tGFP-DTA

   Plasmid

   #161750

   Purpose

   tGFP plasmid for the c-terminal targeting of human GSX2 locus

   Depositor

   Elena Cattaneo

   Article

   Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

   Insert

   turboGFP
   

   Use

   CRISPR

   Tags

   P2A-tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   endogenous GSX2 promoter

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

   Plasmid

   #115432

   Purpose

   Vector for expressing RPL28 with mutagenized exon2

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   exon 2 deleted, with MfeI / NheI cloning site

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p.UTA.2.0-GL-2-3 perfect copies

   Plasmid

   #82441

   Purpose

   Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mutation

   The complementary region for GL2 siRNA control wa…

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19/PLIN2-GFP

   Plasmid

   #202194

   Purpose

   knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP knock-in

   Expression

   Mutation

   Promoter

   lac

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-sp-myc-mSema4D

   Plasmid

   #190645

   Purpose

   Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

   Depositor

   Andrew Newman, Victor Tarabykin

   Article

   Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

   Insert

   Sema4d (Sema4d Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHiUGE TurboID-HA donor (all-in-one) ORF0

    Plasmid

    #200383

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAM1

    Plasmid

    #51401

    Purpose

    Expresses truncated version of NLL-MetRS from endogenous MetG promoter

    Depositor

    David Tirrell

    Article

    Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

    Insert

    MetRS (metG E coli)
    

    Use

    Tags

    Expression

    Mutation

    L13N, Y260L, H301L; Please note that a truncated …

    Promoter

    MetG

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

    Plasmid

    #162164

    Purpose

    Selectable homology donor template for Aag2 cell AGO1

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    RFP
    3xFLAG
    

    Use

    CRISPR and Cre/Lox; Homology donor template

    Tags

    AGO1 homology and loxP

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and endogenous AGO1

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHiUGE TurboID-HA donor (all-in-one) ORF2

    Plasmid

    #200385

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHiUGE TurboID-HA donor (all-in-one) ORF1

    Plasmid

    #200384

    Purpose

    Tagging endogenously expressed proteins with TurboID-HA, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBrain-GFP-FKBP-TACC3-shTACC3

    Plasmid

    #59354

    Purpose

    Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    EGFP and FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHiUGE smFP-V5 donor (all-in-one) ORF0

    Plasmid

    #200386

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHiUGE smFP-V5 donor (all-in-one) ORF1

    Plasmid

    #200387

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid

    #59356

    Purpose

    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IR25a-T2A-QF-AD

    Plasmid

    #162526

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHiUGE smFP-V5 donor (all-in-one) ORF2

    Plasmid

    #200388

    Purpose

    Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

    Insert

    spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

    Plasmid

    #176542

    Purpose

    Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Use

    Tags

    3' extension derived from endogenous locus a…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Use

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. RepairTemplate_Smc4-mAID-HaloTAG

    Plasmid

    #217658

    Purpose

    Repair template to endogenously tag SMC4 with mAID-Halo Tag

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    SMC4-mAID-HaloTag (SMC4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ORCO-T2A-QF-DBD

    Plasmid

    #162525

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pIS1-Vim/Actb5UTR-renilla

    Plasmid

    #38238

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Actb promoter (1000 nt upstream) and 5' UTR …

    Promoter

    Endogenous Vim

    Available since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid

    #140228

    Purpose

    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    RRM2 endogenous promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Th-iCre

    Plasmid

    #159459

    Purpose

    Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

    Depositor

    Yuejun Chen

    Article

    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

    Insert

    P2A-iCre
    

    Use

    Cre/Lox

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous TH

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pONSY-CoH2B:Venus

    Plasmid

    #111877

    Purpose

    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora Histone H2B (CoH2B) fused to Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ORCO-T2A-QF2

    Plasmid

    #162524

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p.UTA.2.0 miR-27a-3p 3 perfect copies.

    Plasmid

    #82439

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Gr4-T2A-QF2 HDR plasmid

    Plasmid

    #140944

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

    Depositor

    Leslie Vosshall

    Article

    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

    Insert

    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Sdk1-AP-His

    Plasmid

    #72008

    Purpose

    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sdk1 (Sdk1 Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Sdk1-Fc-His

    Plasmid

    #72134

    Purpose

    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sdk1 (Sdk1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RF-118-UB-BRCA1

    Plasmid

    #78960

    Purpose

    Expression of the C-terminal fragment of BRCA1 that is endogenously generated by caspase cleavage in mammalian cells

    Depositor

    Richard Fahlman

    Article

    Xu et al J Biol Chem. 2012 Mar 2;287(10):7495-502

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    3x Flag and ubiquitin

    Expression

    Mammalian

    Mutation

    delta 1-1155

    Promoter

    CMV

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p2701-AGER-tdTomato

    Plasmid

    #216470

    Purpose

    donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Insert

    tdTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pGL4.23-Gja1prom

    Plasmid

    #188114

    Purpose

    Expresses luciferase under control of Gja1 promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

    Insert

    Gja1 promoter (Gja1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Gja1 endogenous promoter up to gene start codon

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. IR76b-T2A-QF2

    Plasmid

    #162523

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. GR3-T2A-QF2

    Plasmid

    #162521

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. IR8a-T2A-QF2

    Plasmid

    #162520

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid

    #184774

    Purpose

    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pNHT7::UMSBP::sNluc2_ORF2

    Plasmid

    #186766

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon..

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. phABCA3-eGFP_CRISPR_Donor

    Plasmid

    #188540

    Purpose

    Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    EGFP
    

    Use

    CRISPR; Donor for homologous recombination

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. IR25a-T2A-QF2

    Plasmid

    #162522

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pORANGE_NFL linker-3xFLAG KI

    Plasmid

    #182679

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid

    #182680

    Purpose

    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge