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Plasmid#173867PurposeMammalian expression of mVenus-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)DepositorInsertmVenus-NES-SspB-SOS2cat-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173875PurposeMammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax
Plasmid#173876PurposeMammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)DepositorInsertmCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer21 Flag-Sesn2 419A/422A/426A
Plasmid#61870PurposeDox inducible lentivirial expression of Flag-Sesn 419A/422A/426A (mouse) mutantDepositorInsertSesn2 (Sesn2 Mouse)
UseTagsFlagExpressionMammalianMutation419A/422A/426APromoterCMVAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
Plasmid#173871PurposeMammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)DepositorInsertmCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PHR-iSH-T2A-CIBNcaax
Plasmid#173860PurposeMammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)DepositorInsertmCherry-PHR-iSH-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
Plasmid#173866PurposeMammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)DepositorInsertmVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
Plasmid#173865PurposeMammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)DepositorInsertmVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM005
Plasmid#170009PurposeEncodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PvirKAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM002
Plasmid#170006PurposeEncodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PpmrDAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMPRA1-CMV-Clover
Plasmid#53538PurposeFluorescent reporter control for pMPRA1DepositorInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMPRA2-CMV-Clover
Plasmid#53539PurposeFluorescent reporter control for pMPRA2DepositorInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMPRA3-CMV-Clover
Plasmid#53540PurposeFluorescent reporter control for pMPRA3DepositorInsertClover GFP
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJTRSF55
Plasmid#89701PurposeFor production of GFP with "GLP" N-tag and "TRNAL" for C-tag for cyclisation control.DepositorInsertSMT3_GLP_H6_GFP_TRNAL
UseTagsSMT3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PAF23
Plasmid#223171PurposeExpresses yeGFP under control of CTR1 promoter and terminatorDepositorInsertgreen fluorescent protein
UseTagsExpressionYeastMutationPromoterSaccharomyces cerevisiae CTR1Available sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pITRSF4
Plasmid#89699PurposeFor production of GFP with "GLP" N-tag and "TRNGL" for C-tag for cyclisation control.DepositorInsertSMT3_GLP_H6_GFP_TRNGL
UseTagsSMT3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJTRSF50
Plasmid#89700PurposeFor production of GFP with "GLP" N-tag and "TRNCL" for C-tag for cyclisation control.DepositorInsertSMT3_GLP_H6_GFP_TRNCL
UseTagsSMT3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM003
Plasmid#170007PurposeEncodes PmgtC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PmgtCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM004
Plasmid#170008PurposeEncodes PugtL-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PugtLAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM006
Plasmid#170010PurposeEncodes PpagC-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PpagCAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAM007
Plasmid#170011PurposeEncodes PslyB-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoPDepositorArticleInsertsSuperfolder GFP
PhoP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1 and PslyBAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Neo-TGmiR-Luc
Plasmid#25745PurposeControl lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.DepositorInsertLuciferase miR-shRNA
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
CRY2-OCRL(D523G)
Plasmid#79567Purposeexpression of CRY2PHR fused to phosphatase dead mutant of OCRLDepositorInsertOCRL (OCRL Human)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539 and phosphatase mutation, D523GPromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-monSTIM1
Plasmid#161795PurposeMammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)DepositorInsertMonSTIM1 (STIM1 Human, Mustard Weed)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gfaABC1D-monSTIM1
Plasmid#161797PurposeLentiviral vector expressing monSTIM1 in astrocytes.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromotergfaABC1DAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIa-monSTIM1
Plasmid#161796PurposeLentiviral vector expressing monSTIM1 in neurons.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCamKIIaAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-TetR
Plasmid#103813PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY9
Plasmid#130907PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-2G6, and PtetAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY59
Plasmid#130927PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).DepositorInsertsdcas9
tetR
sfgfp
pspFΔHTH::MCP
UseSynthetic BiologyTagsASV tag and MCP (MS2 coat protein)ExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-2G6, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY57
Plasmid#130926PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA5B5, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY56
Plasmid#130925PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA4B4, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY55
Plasmid#130924PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA3B3, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY53
Plasmid#130911PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA1B1, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-R/G-ICUE
Plasmid#181849PurposeICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.DepositorInsertR/G-ICUE (RAPGEF3 Human)
UseTagsmRuby2 and sfGFPExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-ICUE
Plasmid#181845PurposeGenetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-ICUE (RAPGEF3 Human)
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-7
Plasmid#199212PurposeLanding Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection markerDepositorInsertsaminoglycoside phosphotransferase,
enhanced Green Fluorescence Protein
Red Fluorescence Protein
UseTagsExpressionMammalianMutationPromoterCMV and thymidine kinase promoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-DIO-mCherry-nega miR
Plasmid#137732PurposeLentivirus vector for control miR in a Cre-dependent mannerDepositorInsertcontrol microRNA
UseLentiviralTagsmChherryExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRV FOXP3 WRR
Plasmid#13624DepositorInsertFOXP3 (FOXP3 Human)
UseRetroviralTagsMycExpressionMammalianMutationWRR: N361W, E399R, E401RPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-44
Plasmid#91780PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP3-44
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
FP5369 KIF17MD-flag- FIREmate
Plasmid#216724PurposeExpresses motor domain of KIF17 fused to FIREmate in mamallian cellsDepositorInsertKIF17 (KIF17 Human)
UseTagsFIREmate and FlagExpressionMammalianMutation1-547PromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cx36-GCaMP
Plasmid#123604PurposeMouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cellsDepositorInsertConnexin 36-GCaMP3 fusion (Gjd2 Mouse)
UseTagsGCaMP3ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
Kif5A-CIBN
Plasmid#102251PurposeExpresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)DepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCIG-VP16–RAR-alpha
Plasmid#80434PurposeExpression of constitutively active human RAR-alpha in chick embryosDepositorInsertRetinoic acid receptor alpha (RARA Human)
UseTagsEGFP and VP16ExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-GCaMP3-373
Plasmid#91779PurposeMammalian expression of low affinity calcium-sensing fluorescent proteinDepositorInsertGCaMP3-373
UseTags6xHis, T7 epitope, and Xpress tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-mCherry-RANK
Plasmid#208612PurposeMammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)DepositorInsertCRY2clust-mCherry-RANK (Cry2 Mustard Weed, Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Zdk1-EB1C
Plasmid#107695PurposeC-terminal half of π-EB1, mCherry-tagged, dissociates from microtubule ends in blue lightDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsZdk1 and mCherryExpressionMammalianMutationEB1 aa 186-268PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
Plasmid#173862PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax (PIP3 marker and opto-PI3K)DepositorInsertAktPH-mCherry-T2A-PHR-iSH-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hPMLIII
Plasmid#103807PurposeBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only