165,313 results

1. RfxCas13d-backbone

   Plasmid

   #165078

   Purpose

   Expression of RfxCas13d sgRNAs from a U6 promoter

   Depositor

   Ling-Ling Chen

   Article

   Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

   Insert

   Scaffold gRNA
   

   Expression

   Mammalian

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV3Tag8li-hHP1BP3-HA

   Plasmid

   #165468

   Purpose

   Human HP1BP3 mammalian expression vector with C-terminal HA tag.

   Depositor

   Martin Dorf

   Article

   Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

   Insert

   Heterochromatin protein 1 binding protein 3 (HP1BP3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA-FLAG-METTL3-APPA

   Plasmid

   #160251

   Purpose

   Express catalytic inactive FLAG-METTL3 in human cells

   Depositor

   Alessandro Fatica

   Article

   Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changes in aa 395–398, DPPW → APPA

   Promoter

   CMV

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-polyQ23-NLS

   Plasmid

   #231891

   Purpose

   Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   HTT partial exon 1 Q23-NLS (HTT Human)
   

   Tags

   EGFP and SV40 NLS

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCVpuro-mTagBFP2

   Plasmid

   #96935

   Purpose

   Retroviral plasmid containing mTagBFP2 for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Monomeric Tag Blue Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ND4.2-Right DdCBE-G1397-C- T1413I-GFP

   Plasmid

   #179686

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_P2A_GFP_ATP5B 3'UTR
   

   Expression

   Mammalian

   Mutation

   T1413I

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pINDUCER dCas9-TET1CD

   Plasmid

   #101921

   Purpose

   The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

   Depositor

   Danwei Huangfu

   Article

   Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

   Insert

   dCas9-TET1CD
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-TetON-mEGFP-MYOD1_AroPERFECT

   Plasmid

   #215620

   Purpose

   For integration of MYOD1 AroPERFECT T2A mEGFP

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   MYOD1 AroPERFECT (MYOD1 Human)
   

   Tags

   T2A mEGFP

   Expression

   Mammalian

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) Flag-His-ATM kd

   Plasmid

   #31986

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM kd (ATM Human)
   

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   3'UTRf 9 nucl/D2870A N2875k. catalytically …

   Available Since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLEX307-SARS-CoV-2-3CL

    Plasmid

    #160278

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelper_ShCAST_sgRNA

    Plasmid

    #127921

    Purpose

    Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
    

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 35S-GAL4BD-GWY/pAM

    Plasmid

    #201233

    Purpose

    Used to produce and express in planta N-terminal fusion proteins with the GAL4 BD

    Depositor

    Michael Ayliffe

    Article

    Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

    Insert

    35S-GAL4BD-GWY
    

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. hKDR

    Plasmid

    #83436

    Purpose

    human VEGFR2 with V5 and tGFP tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Cell Physiol. 2018 Aug 1;31

    Insert

    human VEGFR2 (KDR Human)
    

    Tags

    V5 and turboGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MOM603

    Plasmid

    #133242

    Purpose

    expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

    Depositor

    Shinsuke Niwa

    Article

    Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

    Insert

    kif1a (KIF1A Human)
    

    Tags

    StrepII tag and mScarlet

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn7-160

    Plasmid

    #118572

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-7 asyn A53T

    Plasmid

    #105727

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

    Insert

    alpha-synuclein A53T (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    A53T

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pHCMV_SFV_E1E2

    Plasmid

    #207308

    Purpose

    Mammalian expression of Semliki Forest virus E1E2 protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    SFV_E1E2
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVE13300

    Plasmid

    #137715

    Purpose

    Expresses impLbCas12a in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    impLbCas12a
    

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pL99

    Plasmid

    #78577

    Purpose

    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system

    Depositor

    Xuming Mao

    Article

    Sun et al Annals of Microbiology Dec 2012, Volume

    Type

    Empty backbone

    Use

    Streptomyces expression

    Expression

    Bacterial

    Promoter

    inducible PnitA-NitR system

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #130909

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pmCherry-ATG5-K130R

    Plasmid

    #13096

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5-K130R (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K130R

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTW040

    Plasmid

    #64769

    Purpose

    For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pminiTol2

    Plasmid

    #31829

    Depositor

    Stephen Ekker

    Article

    Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti CMV TRE3G Neo GFP-Progerin

    Plasmid

    #118710

    Purpose

    Lentiviral TET-ON inducible GFP-Progerin

    Depositor

    Tom Misteli

    Article

    Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

    Insert

    GFP-Progerin (LMNA Human)
    

    Use

    Lentiviral; Tet-on inducible

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV TRE3G (TET-ON)

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pTALYM4SpMi-05

    Plasmid

    #47880

    Purpose

    TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pExp-Lipo

    Plasmid

    #129237

    Purpose

    Protein expression in E.coli

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    His and Strep

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pUC18-mini-Tn7T-Gm-lacZ

    Plasmid

    #63120

    Purpose

    mini-Tn7 lacZ transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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