We narrowed to 23,290 results for: CRISPR

1. pCE38

   Plasmid

   #174432

   Purpose

   C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCE41

   Plasmid

   #174433

   Purpose

   C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgTrack-GFP

   Plasmid

   #114012

   Purpose

   Empty sgRNA expression vector with co-expression of TurboGFP reporter

   Depositor

   Charles Rudin

   Article

   Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pXCas9H840A

   Plasmid

   #60900

   Purpose

   Dual Expression Vector for Cas9 H840A nickase and gRNA

   Depositor

   Bruce Conklin

   Article

   Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

   Plasmid

   #124862

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgSlc17a8

   Plasmid

   #124848

   Purpose

   Mutagenesis of Slc17a8

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a8 (Slc17a8 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nanog-2A-mCherry

   Plasmid

   #48680

   Purpose

   For knock-in donor vector by CRISPR/CAS system

   Depositor

   Rudolf Jaenisch

   Article

   Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

   Insert

   Nanog (Nanog Mouse)
   

   Use

   CRISPR

   Tags

   Cherry

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV/3' Box_(GLuc)_TOP1

    Plasmid

    #68434

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-IRES-puro-NLS-NLS-dMisCas13b-3xGFP-NLS

    Plasmid

    #191367

    Purpose

    Overexpression of NLS-NLS-dMisCas13b-3xGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-NLS-dMisCas13b-3xGFP-NLS
    

    Tags

    3xGFP

    Expression

    Mammalian

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC2_4_dCas9_VPR_sgRNA

    Plasmid

    #163709

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-memRFP-3xControlgRNA

    Plasmid

    #224567

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM355

    Plasmid

    #173175

    Purpose

    (Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    gRNA-12xMBS backbone
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNHEJ-recAmu-sacB

    Plasmid

    #158717

    Purpose

    CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    recA
    

    Expression

    Bacterial

    Mutation

    R61C

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh22

    Plasmid

    #106695

    Purpose

    expression of gRNA targeting COSMC

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    COSMC
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pPPC019.AAV

    Plasmid

    #171147

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. p15a-AsCas12f-apmR

    Plasmid

    #171610

    Purpose

    AsCas12f1-based K. pneumoniae genome editing

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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