We narrowed to 16,821 results for: IGH@
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pCDFNKI-his-3C-LIC-strep
Plasmid#108705PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneUseTagsHis-3C protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector
Plasmid#132560PurposeVector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.DepositorInsertsABEmax
sgRNA, with Tetraloop replaced by com aptamer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermQ7
Plasmid#51443PurposeExpresses TALEN targeting right CCR5A site (R18) with Q7 C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Q7 C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK777Q, K778Q, K788Q, R789Q, R792Q, R793Q and R801QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluf
Plasmid#105833PurposeFast indicator for extracellular glutamate imagingDepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DNAJC5 (WT)-HaloTag
Plasmid#205710PurposeMammalian expression of DNAJC5 (WT)-HaloTagDepositorInsertDNAJC5 (DNAJC5 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct11
Plasmid#28204DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa193-274 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-StefinA
Plasmid#182403PurposeBacterial expression of Vesicle Nucleating peptide-stefinA fusionDepositorInsertVNp-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D2s-L-V2
Plasmid#19968DepositorInsertD2 short variant-C terminal Venus (DRD2 Aequorea Victoria, Human)
UseTagsC terminal Venus and FlagExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i1 sgRNA / hSpCas9
Plasmid#172825PurposeMammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP2372
Plasmid#73196PurposeHuman expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationR661A, Q695A, and Q926A in SpCas9PromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
MBC1-PuroIII
Plasmid#153419PurposeMSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genesDepositorInsertmurine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-N1-Lyn11
Plasmid#202683PurposePhotoactivatable lipid biosensorDepositorInsertLYN(1-11):PAmCherry1 (LYN Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Spc72
Plasmid#51582PurposeConstitutively express spindle pole body localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSpc72 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mRFP1 gRNA
Plasmid#117052PurposeIn vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoterDepositorInsertanti-mRFP1 SpCas9 gRNA
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNAT3_G-alpha
Plasmid#109948PurposeProtein expression and purification of GNAT3_G-alphaDepositorInsertGNAT3_G-alpha (GNAT3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
UseTagsExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
mRuby2-H2B-6
Plasmid#55897PurposeLocalization: Nucleus/Histones, Excitation: 559, Emission: 600DepositorInsertH2B (H2BC11 Human)
UseTagsmRuby2ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceMarch 4, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBT3019
Plasmid#122541PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2DepositorInsertPnhx-2_CemOrange2::SKL
UseTagsSKLExpressionBacterialMutationPromoterPnhx-2Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Clathrin-15
Plasmid#57947PurposeLocalization: Clathrin Vesicles, Excitation: 549, Emission: 565DepositorInsertClathrin (CLTB Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA - Exon9 E545K (pFastBac)
Plasmid#16640DepositorInsertPIK3CA E545K (PIK3CA Human)
UseBaculoviralTagsHisExpressionMammalianMutationE545KPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ269C2
Plasmid#164716PurposeGateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editingDepositorInserthAID-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible haid-cbe_v04 entry clo…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PRKACA_C1+C2
Plasmid#110027PurposeProtein expression and purification of PRKACA_C1+C2DepositorInsertPRKACA_C1+C2 (PRKACA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-mSOG-bFos-IRES-mCherry
Plasmid#136599PurposeEncodes bFos domain tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).DepositorInsertN-mSOG-bFos
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RAC1_Ras
Plasmid#110030PurposeProtein expression and purification of RAC1_RasDepositorInsertRAC1_Ras (RAC1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
tdp43-EGFP construct10
Plasmid#28203DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsEGFPExpressionMammalianMutationaa1-105 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
Ai39
Plasmid#34884DepositorInsertCAG-Floxed eNpHRv3.0-EYFP
UseMouse TargetingTagsEYFPExpressionMutationPromoterCAGAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
ELF1_pet28a
Plasmid#131649PurposeBacterial expression of ETS gene ELF1DepositorInsertELF1 (ELF1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only
