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We narrowed to 59,739 results for: Gene

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Showing: 10891 - 10920 of 59739 results

  2. pINER5

    Plasmid
    #185895
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 25, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185895-plasmid-map-sequence-id-370388

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  5. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

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  8. pZH519

    Plasmid
    #102672
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102672-plasmid-map-sequence-id-199600

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  9. pZH522

    Plasmid
    #102675
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102675-plasmid-map-sequence-id-199391

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  10. pPMVAd36

    Plasmid
    #98302
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
    Expression
    Yeast
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    98302-plasmid-map-sequence-id-190207

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  13. pBALU12

    Plasmid
    #69328
    Purpose
    Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOP
    Depositor
    Oliver Hobert
    Article
    Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/
    Insert
    gSL2-NLS-mChOint-FgF
    Expression
    Bacterial
    Available Since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. SNV-gfp-IRES-MCS

    Plasmid
    #53122
    Purpose
    Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
    Depositor
    Jeanette Hyer
    Article
    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1
    Type
    Empty backbone
    Use
    Retroviral; For expression in chicken cells
    Tags
    EGFP-IRES
    Promoter
    SNV (spleen necrosis virus)
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    53122-plasmid-map-sequence-id-82652

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  20. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

    Plasmid
    #188385
    Purpose
    Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188385-plasmid-map-sequence-id-371050

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  29. lenti SYN-SVI-DIO-dCas9-VPR

    Plasmid
    #164576
    Purpose
    Expresses a SYN-driven, intron-containing dCas9-VPR fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.
    Depositor
    Jeremy Day
    Article
    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21
    Insert
    A DIO FLAG-dCas9-VPR cassette containing an SV40 intron
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    164576-plasmid-map-sequence-id-323657

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  30. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139087-plasmid-map-sequence-id-273883

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Showing: 10891 - 10920 of 59739 results