We narrowed to 12,778 results for: NUC

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mEmerald-MCM6-N-22

Plasmid

#54172

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM6

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mOrange-H2B-6

Plasmid

#57942

Purpose

Localization: Nucleus/Histones, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Insert

H2B (H2BC11 Human)

Tags

mOrange

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pCVL SFFV HA.NLS.I-AniIY2K227M(reo).T2A.TagBFP

Plasmid

#31478

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV HA NLS Y2 K227M T2A BFP

Use

Lentiviral

Tags

HA and NLS

Mutation

F13Y, S111Y, K227M = Y2 nickase "reo" …

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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3xFNLDD/pMXs-IG

Plasmid

#48972

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

LTR

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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MTK3_008

Plasmid

#123723

Purpose

Encodes nuclear localized bacterial EL222 fused to activating domain of VP16 as Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS-VP16-EL222

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pKM263-EcPPXc

Plasmid

#38328

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

6xHIS, GST, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-NES-MBP-cATOM(Ras)

Plasmid

#209711

Purpose

Mammalian expression of c-ATOM biosensor for HRas with nuclear export signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NES-MBP-cATOM(Ras)

Tags

NES-MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBS/KS-mId2sp

Plasmid

#16044

Depositor

Robert Benezra

Article

Jen et al Dev Dyn. 1996 Nov . 207(3):235-52.

Insert

Id2 (Id2 Mouse)

Expression

Bacterial

Mutation

cDNA nucleotides 1-195

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
mEos2-H1-10

Plasmid

#57383

Purpose

Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

H1 (H1f0 Mouse)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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3xFNLDD/pCMV-7.1

Plasmid

#48874

Purpose

Expresses 3xFNLDD in mammalian cells

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2015 Jan 21;10(1):e0116579. do

Insert

3xFNLDD

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pMEH294 NLS dTomato 1x high affinity MS2 loop

Plasmid

#168213

Purpose

Mammalian expression of nuclear-localized dTomato with 1 high affinty MS2 loop

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

Plasmid

#183803

Purpose

AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

E2-Crimson-NLS

Use

AAV

Tags

NLS

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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Myog'-2A-mCherryNLS-PGK-Purodtk

Plasmid

#69547

Purpose

Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

Depositor

Charles Gersbach

Inserts

2A-mCherry-NLS
Myogenin 5' homology arm (Myog Mouse)
Myogenin 3' homology arm (Myog Mouse)
Puro-delta-tk

Use

Mouse Targeting

Tags

2x nuclear localization signal and PVT1-2A "…

Mutation

Silent V205V mutation in myogenin gene to prevent…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pX330(puro) Rosa26-H3F3B

Plasmid

#73131

Purpose

Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

spCas9

Tags

Flag

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pAT15483_e-ABE8e

Plasmid

#174121

Purpose

Expresses ABE8e fused with eCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

eABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Promoter

EF1-a

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA

Plasmid

#86984

Purpose

Expression of mouse Trex2

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Insert

mouse Trex2 coding region (Trex2 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-NLS-ZapCmR1.1

Plasmid

#59011

Purpose

Nuclear zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NLS-ZapCmR1.1 genetically encoded Zn(II) sensor

Tags

SV40 NLS

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE662

Plasmid

#153260

Purpose

M6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE664

Plasmid

#153262

Purpose

M7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE659

Plasmid

#153257

Purpose

M4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE661

Plasmid

#153259

Purpose

M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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EC0_2_VP64

Plasmid

#163714

Purpose

Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

VP64 + SV40 NLS

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-YFP-NLS

Plasmid

#145274

Purpose

Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MCS and YFP

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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ptCMV-153/47-VR-NG

Plasmid

#50704

Purpose

Final destination vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

TALEN-FokI nuclease backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-C

Plasmid

#170154

Purpose

AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

RESCUE-DDC-λN-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGK415-phlTA2-1E

Plasmid

#165970

Purpose

Expresses phlTA(2-1E) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

phlF

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

P5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…

Promoter

pPGK1 mutant

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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