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Showing: 1441 - 1480 of 30298 results

  2. pBabe puro IRES-EGFP

    Plasmid
    #14430
    Depositor
    L. Miguel Martins
    Has Service
    Cloning Grade DNA
    Insert
    IRES-EGFP
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    14430-plasmid-map-sequence-id-251302

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  6. qTAG-AAVS1-PGK-Puro

    Plasmid
    #227269
    Purpose
    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227269-plasmid-map-sequence-id-452020

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  7. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  10. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8

    Plasmid
    #71828
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA for targeted DNA methylation of BACH2 promoter in human cells; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71828-plasmid-map-sequence-id-131023

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  18. pBabe-puro Ras V12

    Plasmid
    #1768
    Depositor
    Bob Weinberg
    Insert
    ras V12 (HRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    G12V, constitutively active
    Promoter
    Available since
    June 3, 2005
    Availability
    Academic Institutions and Nonprofits only
    1768-plasmid-map-sequence-id-162793

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  31. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid
    #176932
    Purpose
    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_mNG_hSLBP(18-108)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK1
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    176932-plasmid-map-sequence-id-378103

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  34. pWPI-IRES-Puro-Ak-ACE2

    Plasmid
    #154985
    Purpose
    Lentiviral vector expressing Human ACE2
    Depositor
    Sonja Best
    Insert
    ACE2 (ACE2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    154985-plasmid-map-sequence-id-305311

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  38. qTAG-N-Puro-mNeon-ACTB

    Plasmid
    #207752
    Purpose
    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    207752-plasmid-map-sequence-id-414897

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  39. qTAG-N-Puro-sTagRFP-TUBA1B

    Plasmid
    #207769
    Purpose
    Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207769-plasmid-map-sequence-id-413794

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Showing: 1441 - 1480 of 30298 results