Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 721 - 740 of 18759 results

  2. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Publication
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60847-plasmid-map-sequence-id-197511

    Enlarge

  3. pBS-Hsp70-Cas9

    Plasmid
    #46294
    Purpose
    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1
    Insert
    codon optimized Cas9
    Use
    CRISPR
    Tags
    3x Flag
    Expression
    Insect
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Hsp70
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46294-plasmid-map-sequence-id-332831

    Enlarge

  7. pX330 p53

    Plasmid
    #59910
    Purpose
    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting p53 (Trp53 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59910-plasmid-map-sequence-id-516810

    Enlarge

  8. 3xFLAG-dCas9/pCMV-7.1

    Plasmid
    #47948
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    3xFLAG-dCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A (catalyticall…
    Promoter
    CMV
    Available since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    47948-plasmid-map-sequence-id-68812

    Enlarge

  9. XW52

    Plasmid
    #65838
    Purpose
    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method
    Depositor
    Kang Shen
    Publication
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    Czipper-QF-AD-NLS
    Tags
    mCherry
    Expression
    Worm
    Promoter
    Pmig-13
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65838-plasmid-map-sequence-id-120568

    Enlarge

  11. Puro-Cas9 donor

    Plasmid
    #58409
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus
    Depositor
    Danwei Huangfu
    Publication
    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58409-plasmid-map-sequence-id-392399

    Enlarge

  13. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS, No promoter, and U6
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60224-plasmid-map-sequence-id-418949

    Enlarge

  17. pMH0006

    Plasmid
    #135448
    Purpose
    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.
    Depositor
    Martin Kampmann, Jonathan Weissman
    Publication
    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094
    Insert
    dCas9-BFP-KRAB
    Use
    Lentiviral
    Tags
    tagBFP
    Expression
    Mammalian
    Promoter
    EF1A
    Available since
    July 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135448-plasmid-map-sequence-id-304832

    Enlarge

  18. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Publication
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117699-plasmid-map-sequence-id-368109

    Enlarge

Showing: 721 - 740 of 18759 results