We narrowed to 6,501 results for: nls
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Plasmid#12180DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMEKK1 (Map3k1 Mouse)
TagsHis and XpressExpressionMammalianMutationKinase dead: K1253M; V1467LAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-dCas9-PBE
Plasmid#98162PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseRiceExpressionPlantPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTORCAR-NLS (EIF4EBP1 Human, Synthetic)
TagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE7-SB-NLS
Plasmid#228870PurposePE7 connected to MLH1-SB-NLS via 2A sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPE7-2A-MLH1-SB-NLS
UseCRISPRAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP-Cre (JDW 1329)
Plasmid#224515PurposeA Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusionDepositorInsertnls-EGFP-Cre
UseCre/LoxAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pW193-lenti-sasgRNA-lacZ-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170814PurposeLentiviral vector to co-express a lacZ control sasgRNA with NLS-mNeonGreenDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW301-lenti-spsgRNA-hsITGB1-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170817PurposeLentiviral vector to co-express a human ITGB1 spsgRNA with NLS-mScarlet-IDepositorInsertNLS-mNeonGreen-P2A-BlastR
UseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9::BFP-humanized
Plasmid#44247PurposeExpression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdCas9 fused to BFP
UseCRISPR and RetroviralTags2xNLS & tagBFPExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailable sinceApril 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TC513
Plasmid#122616PurposePunc-4c::SV40 NLS::rtetR::Zip(+)::SL2::mCherryDepositorInsertSV40 NLS::rtetR::Zip(+)::SL2::mCherry
ExpressionWormAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSYC-102
Plasmid#74790PurposepCS4+-NLS-EGFP-T2A-mCherry-CAAXDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-EGFP-T2A-mCherry-CAAX
Available sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-Jmjd3-F
Plasmid#17440PurposeExpression of Jmjd3 in mammalian cellsDepositorAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ166-iSpyMac
Plasmid#149553PurposeGateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMsDepositorInsertiSpyMacCas9
UseCRISPR; Gateway compatible zispymaccas9 entry clo…Tags3X FLAG, NLS and NLSExpressionPlantMutationR221K, N394K, Protospacer interacting domain form…Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and pTRE3G-bidirectionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP441
Plasmid#158801PurposePlasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCasPhi-2
Tags2xSV40NLS-2xFLAGExpressionMammalianAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRD190
Plasmid#163100PurposeExpression of mEos3.2 in eukaryotes to stain the nuclear compartmentDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS_mEos3.2_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L1-dMb2Cas12a
Plasmid#177683PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeExpressionPlantPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
L1_ARF19_2
Plasmid#195914PurposeLevel 1 PhiC31 cassette with LR dvp promoter: pARF19-PhiC31-NLS-tUBQ1DepositorInsertpARF19-PhiC31-NLS-tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1P2
Plasmid#195918PurposeLevel 1 PhiC31 cassette with constitutive promoter: pPP2AA3-PhiC31-NLS-tUBQ1DepositorInsertpPP2AA3-PhiC31-NLS-tUBQ1
ExpressionPlantAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only