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20,146 results

Showing: 11221 - 11250 of 20146 results

  1. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Article
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available Since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

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  18. PKM2 (3GQY)

    Plasmid
    #25360
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PKM2_1 (PKM Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. pRCC-N

    Plasmid
    #81192
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81192-plasmid-map-sequence-id-158700

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  23. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Available Since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107024-plasmid-map-sequence-id-208524

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  25. pRK5-myc-Cdc42-Q61L

    Plasmid
    #12974
    Depositor
    Gary Bokoch
    Insert
    Cdc42 constitutively active (CDC42 Human)
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Q61L Constitutively active
    Available Since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only
    12974-plasmid-map-sequence-id-132746

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Showing: 11221 - 11250 of 20146 results