174,131 results

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STING-miRFP680

Plasmid

#200940

Purpose

STING fused with miRFP680 for live cell imaging

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

STING (STING1 Human)
miRFP680

Use

Lentiviral

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pIVM_02

Plasmid

#204500

Purpose

Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

Depositor

Alessio Ciulli

Article

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

amino acids 54-213 VHL (VHL Human)

Tags

6xHis, TEV, and Thrombin

Expression

Bacterial

Mutation

0

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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NbV5-eGFP

Plasmid

#201473

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-eGFP

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Alpha-Synuclein-His

Plasmid

#186802

Purpose

Codon-optimized bacterial expression plasmid. Expresses Alpha-Synuclein-His.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Alpha-Synuclein-His (SNCA Human)

Tags

His6

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMVmin-luc2

Plasmid

#194384

Purpose

Reporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

6xCRE-CMVmin-luc2

Expression

Mammalian

Promoter

CMV minimal

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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mRNA-CleanCap-mNeonGreen

Plasmid

#188248

Purpose

Template plasmid to transcribe mRNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-anti-AlfaTag nanobody

Plasmid

#171818

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

cfSGFP2-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.iGluSnFR3.v857.PDGFR

Plasmid

#178329

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Has Service

AAV1

Insert

pAAV hSyn iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4.10/RSV_SF3B3-Xon_Luciferase

Plasmid

#174659

Purpose

Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

Depositor

Beverly L. Davidson

Article

Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

Insert

Xon cassette

Use

Luciferase

Expression

Mammalian

Mutation

Kozak ATG initiation sequence in middle exon

Promoter

RSV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSAB35

Plasmid

#175787

Purpose

Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

Depositor

John Rubinstein

Article

Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

Insert

SidK (1-278)

Tags

3xFLAG and 6xHis TEV

Expression

Bacterial

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-His6-2_HBD

Plasmid

#171870

Purpose

Expresses tandem repeats of HBD domain of RNase H1 for better DNA-RNA Hybrid recognition specificity in bacterial cells

Depositor

Kaiwei Liang

Article

Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

Insert

Tandem repeats Hybrid binding domain of Rnase H1

Tags

GST- and His10-tag

Expression

Bacterial

Mutation

WT

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.3_MERS_D12

Plasmid

#170448

Purpose

Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

Depositor

David Nemazee

Article

Rogers et al Science. 2020 Aug 21;369(6506):956-963.

Insert

MERS spike D12 (S MERS)

Expression

Mammalian

Mutation

last 12aa deletion in c-terminal tail

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDONRp5-p2 SspB-mCherry-CShroom3

Plasmid

#170977

Purpose

C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

CShroom3 (Shroom3 Mouse)

Use

Gateway Cloning

Tags

SspB and mCherry

Mutation

Isoform X2, deleted aminoacids 1 - 1388

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Promoter

pB-Globin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166014

Purpose

mammalian expression of HCoV-HKU1 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-HKU1 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSL21-mCherry

Plasmid

#164410

Purpose

Retroviral expression plasmid of sgRNA with mCherry

Depositor

Junwei Shi

Article

Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6 promoter for crRNA expression and EFS promoter…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(Cyto)iGlucoSnFR

Plasmid

#164513

Purpose

Green glucose sensor, cytosolic. The depositing lab recommends the updated pAAV.GFAP.(cyto).iGlucoSnFR2 (Plasmid #244111) instead of this plasmid.

Depositor

Loren Looger

Article

Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi

Insert

(Cyto)iGlucoSnFR

Use

AAV

Mutation

Synthetic Construct

Promoter

GFAP

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Tags

GST

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-mGreenLantern

Plasmid

#161913

Purpose

mGreenLantern fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

6xHis

Expression

Bacterial

Promoter

araB

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pMK395 (AAVS1 CMV-OsTIR1(F74A))

Plasmid

#159992

Purpose

Expression of OsTIR1(F74A) in human cells

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CMV-OsTIR1(F74A)

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gαq*-BERKY3

Plasmid

#158217

Purpose

BRET biosensor for detection of Gαq-GTP in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK2(RH)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-FOXP3

Plasmid

#153147

Purpose

Expression of Flag tagged human FOXP3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP3 (FOXP3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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