19,171 results

1. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGSKU

   Plasmid

   #72243

   Purpose

   Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

   Depositor

   Francesca Storici, Michael Resnick

   Article

   Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

   Inserts

   KlURA3
   kanMX4
   I-SceI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFGET19_Ulp1

   Plasmid

   #64697

   Purpose

   H6-Ulp1 from pHYRS52 into pET-28b(+)

   Depositor

   Hideo Iwai

   Article

   Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

   Insert

   ULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Ulp1(403-621)

   Promoter

   T7

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3/F-tractin=mStayGold

   Plasmid

   #212019

   Purpose

   For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   F-tractin
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-FLEX-RiboL1-jGCaMP8s

   Plasmid

   #192616

   Purpose

   AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   FLEX-RIboL1-jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lamp1_mStayGold

   Plasmid

   #221902

   Purpose

   Expresses mStayGold fused to Lamp1

   Depositor

   Janelia Integrative Imaging

   Insert

   Lamp1_mStayGold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. L4440

   Plasmid

   #1654

   Depositor

   Andrew Fire

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   RNAi

   Tags

   T7p, T7p, lacZN, OriF1>>, OriF1<<

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-Halo tag

    Plasmid

    #154144

    Purpose

    Gateway compatible mammalian expression plasmid for halo tag fusion proteins

    Depositor

    Michael Huen

    Type

    Empty backbone

    Use

    Tags

    Halo tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCAS9-VP64_GFP

    Plasmid

    #61422

    Purpose

    Expresses dCAS9-VP64 activator with 2A GFP

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Concentrated Lentiviral Prep

    Insert

    dCAS9(D10A,H840A)-VP64_2A_GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    EF1A

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG RABV-G

    Plasmid

    #36398

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    rabies virus glycoprotein
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGs

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGEN-luxCDABE

    Plasmid

    #44918

    Depositor

    Harry Mobley

    Article

    Lane et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    sigma 70 promoter
    

    Use

    Luciferase

    Tags

    lux transcriptional fusion

    Expression

    Mutation

    Promoter

    em7

    Available since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGP-CMV-GCaMP6f

    Plasmid

    #40755

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6f
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-N1-PARP1

    Plasmid

    #211578

    Purpose

    CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKO403_lacZ_Sp

    Plasmid

    #174726

    Purpose

    Bifidobacterium-E.coli shuttle vector for seamless gene cloning

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPSU150

    Plasmid

    #173836

    Purpose

    Expresses 150 kD Penn State ladder protein

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABMBPpepN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. phU6-gRNA

    Plasmid

    #53188

    Purpose

    Expresses the S. pyogenes sgRNA from the human U6 promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-mCherry

    Plasmid

    #36084

    Purpose

    3rd generation lentiviral plasmid expressing mCherry

    Depositor

    Pantelis Tsoulfas

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUGW-PercevalHR

    Plasmid

    #49083

    Purpose

    Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-FLEx-ER-TurboID

    Plasmid

    #160857

    Purpose

    Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p21-Reporter

    Plasmid

    #52432

    Purpose

    DNA damage inducible Red Fluorescent Protein for mammalian expression

    Depositor

    Joel Voldman

    Article

    Fendyur et al Anal Chem. 2014 Aug 5;86(15):7598-605. d

    Insert

    p21-p53 Tandem Response Element Promoter
    

    Use

    Tags

    TurboRFP

    Expression

    Mammalian

    Mutation

    Promoter

    13X repeated p21-p53 response element

    Available since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA-HA-ER WT

    Plasmid

    #49498

    Purpose

    Expresses HA-tagged ER wild-type in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HA-HIF1alpha-pcDNA3

    Plasmid

    #18949

    Depositor

    William Kaelin

    Article

    Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFPC1-huNFATc1EE-WT

    Plasmid

    #24219

    Depositor

    Jerry Crabtree

    Article

    Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MI-Luciferase-IRES-mCherry

    Plasmid

    #75020

    Purpose

    Encodes the expression of Luciferase and mCherry

    Depositor

    Xiaoping Sun

    Article

    Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

    Inserts

    firefly Luciferase
    mCherry
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge