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Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only
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pFA6a-FLAG-APEX2-NES-TRP1
Plasmid#229228PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:H2B-mGreenLantern
Plasmid#230045PurposeExpression of nuclear green fluorescent protein in zebrafish hepatocytes.DepositorInsertmGreenLantern
UseZebrafish expressionTagsH2BExpressionMutationPromoterfabp10aAvailable sinceFeb. 5, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACYCDuet-1:ChrA-C63S-C66S
Plasmid#225082PurposeExpresses precursor peptide ChrA-C63S/C66S in E.coliDepositorInsertChrA-C63S/C66S
UseTagsHis6ExpressionBacterialMutationC63S/C66SPromoterT7Available sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C66S
Plasmid#225081PurposeExpresses precursor peptide ChrA-C66S in E.coliDepositorInsertChrA-C66S
UseTagsHis6ExpressionBacterialMutationC66SPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM59
Plasmid#227655PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, Δ78–81PromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM57
Plasmid#227654PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, V80APromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM54
Plasmid#227653PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, L132APromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C63S
Plasmid#225080PurposeExpresses precursor peptide ChrA-C63S in E.coliDepositorInsertChrA-C63S
UseTagsHis6ExpressionBacterialMutationC63SPromoterT7Available sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-1:ChrH-ChrI
Plasmid#225083PurposeExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coliDepositorInsertChrH
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
rSirt3 shRNA (pLKO.1 vector)
Plasmid#216807PurposeshRNA against rat Sirt3DepositorInsertSirt3 (Sirt3 Rat)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-SHmOCR
Plasmid#206874PurposeEmpty Gateway pDEST vector for bacterial expression with Super His - Monomeric OCR fusionDepositorTypeEmpty backboneUseTagsHSV + 6xHis and SuperHis (14xHis) + monomeric OCR…ExpressionBacterialMutationPromoterT7Available sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
185_pETcon_SARS2_BQ1-1
Plasmid#208085Purposeyeast surface display of the SARS-CoV-2 BQ.1.1 variant RBDDepositorInsertSARS-CoV-2 BQ.1.1 RBD (S SARS-CoV-2 virus, Budding Yeast)
UseTagsHA and c-MycExpressionYeastMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_RV-B14_VP1
Plasmid#202074PurposeBacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-B14 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_RV-A89_VP1
Plasmid#202073PurposeBacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-A89 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_E30_VP1
Plasmid#202070PurposeBacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertE30 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVB1_VP1
Plasmid#202069PurposeBacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVB1 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_CVA4_VP1
Plasmid#202068PurposeBacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertCVA4 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNL2114 pARF19 GG0-GG1
Plasmid#195909PurposeLevel 0 pARF19 promoter with GG0 and GG1 golden gate linkersDepositorInsertpARF19 (ARF19 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I16 tUBQ1 GG5-GG6
Plasmid#195898PurposeLevel 0 tUBQ1 terminator with GG5 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I15 tUBQ1 GG4-GG6
Plasmid#195897PurposeLevel 0 tUBQ1 terminator with GG4 GG6 golden gate linkersDepositorInserttUBQ1 (UBQ1 Mustard Weed)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV puro PRDM14
Plasmid#193677PurposeConstitutive retroviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX304 PRDM14
Plasmid#193688PurposeConstitutive lentiviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mCherry-HRasCT
Plasmid#186704PurposeEncoding membrane-targeted mCherryDepositorInsertmCherry-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB221
Plasmid#185071PurposeSST::ACT2 "SA236"DepositorInsertACT2
UseTagsExpressionYeastMutationACT2-SA236PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB215
Plasmid#185068PurposeSST::WAK2 EcoRI/ScaI fragment (secretion confirmed)DepositorInsertWAK2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-MBP-bhaB5
Plasmid#167812PurposeExpresses His6- and MBP-tagged BhaB5 in E. coliDepositorInsertBhaB5
UseTagsHis6, MBPExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-Daxx KO sgRNA
Plasmid#186939PurposeDaxx KO in mouse ES cellsDepositorInsertDaxx KO sgRNA (Daxx Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMammalianMutationPromoterU6Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Plekhh1 5 silent mutations
Plasmid#187373PurposeExpresses human Plekhh1 with 5 silent mutations labelled with CherryDepositorInsertPlekhh1 with 5 silent mutations
UseTagsmCherryExpressionMammalianMutationFive silent mutations at the Plekhh1 siRNA site (…PromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.HA.HA.Mm.RAP1.I312R
Plasmid#184643PurposeRetroviral expressions of mouse RAP1 I312RDepositorInsertrap1
UseRetroviralTagsHA FLAGExpressionMammalianMutationI312RPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEGF-exon1
Plasmid#177261PurposeA knockout vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEGF-5’UTR
Plasmid#177260PurposeA knockout vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2Aneo-mRFP-FKBP-mSos1-linkercat
Plasmid#173858PurposeEncoding a part of the rapamycin-induced Ras activation system.DepositorInsertmRFP-FKBP-mSos1-linkercat
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EBFP_Nick_Dual_sgRNA
Plasmid#178094PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EBFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EGFP_Nick_Dual_sgRNA
Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dNrg1
Plasmid#173696PurposeA knockout vector for the dog Nrg1.DepositorInsertA gRNA targeting the dog Nrg1 gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEreg
Plasmid#173697PurposeA knockout vector for the dog Ereg.DepositorInsertA gRNA targeting the dog Ereg gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dTgfa
Plasmid#173698PurposeA knockout vector for the dog Tgfa.DepositorInsertA gRNA targeting the dog Tgfa gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dHbegf
Plasmid#173699PurposeA knockout vector for the dog Hbegf.DepositorInsertA gRNA targeting the dog Hbegf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX459-dEgf
Plasmid#173700PurposeA knock-out vector for the dog EgfDepositorInsertA gRNA targeting the dog Egf gene and the cDNA of CRISPR-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only