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pcDNA3.2/pr-Membrane

Plasmid

#120817

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

pr-Membrane ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Promoter

Endogenous T/brachyury

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Dest47- C11orf83-GFP

Plasmid

#65844

Purpose

Mammalian expression of C terminally GFP-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-p53P275S

Plasmid

#22726

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

P275S

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-CUL1 delta N53

Plasmid

#19950

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

cullin 1 delta N53 (CUL1 Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of 53 residues from the NH2-terminus(de…

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pTol2pA2-mnx1:MLS-KillerRed

Plasmid

#115515

Purpose

Plasmid driving expression of membrane bound KillerRed fluorophore in zebrafish motor neurons

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway cloning

Tags

MLS

Promoter

-3mnx1

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-tagRFP-Mad2

Plasmid

#114021

Purpose

Lentiviral delivery of tagRFP-MAD2 expressed from the CMV promoter

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

tagRFP-Mad2 (MAD2L1) (MAD2L1 Human)

Use

Lentiviral

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-miRE_shBRD9_561-SV40-GFP

Plasmid

#75139

Purpose

Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_561

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
p3xFlag-CMV7-Erk2

Plasmid

#39223

Depositor

Melanie Cobb

Article

Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

Insert

Erk2 (Mapk1 Rat)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Mutation

OsTIR1 codon optimized for human cells

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEN716 - pCAGGS-eGFP-3Xnls-bpA

Plasmid

#156435

Purpose

For transient expression of nuclear eGFP in mammalian cells

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

eGFP

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTK47_mCherry-LGN-hardened

Plasmid

#46347

Purpose

rescues the phenotypes caused by LGN RNAi

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

LGN (GPSM2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-6

Plasmid

#72346

Purpose

Mammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Gal4-PGC1 alpha

Plasmid

#8892

Depositor

Bruce Spiegelman

Article

Puigserver et al Cell 1998 Mar 20;92(6):829-39.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only
CMV-tdTomato-P2A-paCaMKII(K42M)

Plasmid

#165433

Purpose

Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(K42M)

Tags

tdTomato

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS5

Plasmid

#120825

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS5 ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

Plasmid

#120555

Purpose

Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

Depositor

Yu Wang

Article

Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

Insert

Cas9, NES, ERT2 and GCN4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-Y207F+W275L

Plasmid

#196022

Purpose

Expresses CRKL Y207F + W275L

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pWzl_CRKL-W275L

Plasmid

#196019

Purpose

Expresses CRKL W275L

Depositor

William Hahn

Article

Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

Insert

CRKL

Use

Retroviral

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-myosin Vb tail 1436-1846

Plasmid

#162714

Purpose

Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

Myosin Vb (Myo5b Rat)

Tags

GFP

Expression

Mammalian

Mutation

WT with deletion of the 1435 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1a-EGFP

Plasmid

#205303

Purpose

Expression of WHaloCaMP1a-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

Plasmid

#191628

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CMV-GFP

Plasmid

#83251

Purpose

Adenoviral expression of STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Tags

FLAG

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
Lyn-mCherry-GAI(1-92)

Plasmid

#37315

Depositor

Takanari Inoue

Article

Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

Insert

GAI (GAI Mustard Weed)

Tags

Lyn and mCherry

Expression

Mammalian

Mutation

contains aa 1-92

Promoter

CMV

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only
pICSL12009

Plasmid

#68257

Purpose

Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter and 5UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-BiP-Myc-KDEL T37G

Plasmid

#27165

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Mutation

ATP bound form: T37G

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pLC133

Plasmid

#198639

Purpose

Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

Depositor

David Bikard

Article

Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

Insert

dCas9

Tags

3x-FLAG

Expression

Bacterial

Promoter

pTet

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJS183

Plasmid

#134142

Purpose

Bacterial expression of GFP-Ska1 and Ska2

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-GFP-human Ska1; human Ska2

Tags

GFP

Expression

Bacterial

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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