We narrowed to 164,714 results for: DES

Showing: 7641 - 7660 of 164714 results

Results per page:

Sort results by:

Myr-(iSH2-p85)-p110alpha-Myc

Plasmid

#1410

Depositor

Lewis Cantley

Insert

PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)

Tags

Myc

Expression

Mammalian

Promoter

SRalpha promoter

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV phSyn1-FSF-FLEX-ChR2(H134R)-EYFP-WPRE-bGHpA

Plasmid

#65454

Purpose

Can be used to generate AAV virus that will express the ChR2(H134R)-EYFP channelrhodopsin fusion protein in neurons under intersectional control by Flp and Cre recombinases

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

ChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Mutation

H134R variant

Promoter

hSyn1

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
ePop

Plasmid

#50953

Purpose

Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

Depositor

Lingchong You

Article

Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

Inserts

E lysis protein
LuxI
LuxR

Mutation

Early stop codon introduced in mutated luxR resul…

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Article

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Human, Synthetic, Codon optimised)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-Tet-UPF1WT (pRKB264)

Plasmid

#99146

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1wt

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFPC2-Gephyrin P1

Plasmid

#68815

Purpose

expression of fluorescent tagged Geph in mammalian cells

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyri…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV HA hRb delta CDK

Plasmid

#58906

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LZRS ERTm RasV12

Plasmid

#21199

Depositor

Paul Khavari

Article

Dajee et al Oncogene. 2002 Feb 28;21(10):1527-38

Insert

EsR1- HRas (Esr1, HRAS Mouse, Human)

Use

Retroviral

Mutation

Esr1 G257R; HRasG12V ERTm bx3

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB2-mScarlet

Plasmid

#104006

Purpose

Plasmid encoding mScarlet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
hGFAP-Cre

Plasmid

#40591

Purpose

Mouse astrocyte expression of Cre

Depositor

Albee Messing

Article

Zhuo et al Genesis. 2001 Oct;31(2):85-94.

Insert

Cre recombinase (GFAP Mouse, Human, Phage)

Use

Cre/Lox and Mouse Targeting

Tags

MP-1 fragment (mouse protamine 1 intron and polya…

Expression

Mammalian

Mutation

Construct contains a nuclear-targeted Cre recombi…

Promoter

hGFAP promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaster12

Plasmid

#58527

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 Human)
Myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
NANOG (NANOG Human)
IRES
NR5A2 (NR5A2 Human)
miR302/367 (MIR367, MIR302A Human)

Expression

Mammalian

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF5B-FRT-control-APEX

Plasmid

#73187

Purpose

Expresses "control"-APEX (cytosolic) in mammalian cells

Depositor

Maxence Nachury

Article

Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

Insert

control-APEX (Nphp3 Glycine max, Mouse)

Tags

internal GFP tag

Expression

Mammalian

Mutation

changed glycine 2 to alanine

Promoter

EF1alpha

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPT-nlsLacZ

Plasmid

#12261

Depositor

Didier Trono

Insert

LacZ

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pX165-HiFi Cas9

Plasmid

#140563

Purpose

Expresses HiFi Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

R691A

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge