We narrowed to 25,556 results for: lars

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pHH0103_UEVLD_UEV

Plasmid

#109902

Purpose

Protein expression and purification of UEVLD_UEV

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UEVLD_UEV (UEVLD Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_RAB5A

Plasmid

#111692

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

RAB5A (RAB5A Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-L1PA4CHmneo

Plasmid

#69607

Purpose

Expresses a neomycin tagged codon optimized L1PA4

Depositor

Astrid Roy-Engel

Article

Wagstaff et al Mol Biol Evol. 2013 Jan;30(1):88-99. doi

Insert

L1PA4

Tags

mneo retrotransposition cassette

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD

Plasmid

#127783

Purpose

Expresses SsCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0510

Plasmid

#124001

Purpose

Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEBB-BT-Ubiquitin

Plasmid

#36098

Depositor

Ezra Burstein

Article

Maine et al Nat Protoc. 2010 Aug;5(8):1447-59. Epub

Insert

Ubiquitin (RPS27A Human)

Tags

TB-tag

Expression

Mammalian

Promoter

EF1a

Available Since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

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MTK678_002

Plasmid

#123923

Purpose

Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BAC-AmpR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEBTet-p52-HDM2_sensor

Plasmid

#127184

Purpose

Eukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interaction

Depositor

Kai Johnsson

Article

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

p53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2

Tags

HisTag and StrepTag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_YTHDF1-Halo

Plasmid

#175301

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

YTHDF1 (YTHDF1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK300

Plasmid

#110592

Purpose

BTK Type 4 terminator for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

rpoC

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BC-A1-001

Plasmid

#78688

Purpose

A strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pSCALPS_miR-146aSponge_mCherry_GFP

Plasmid

#149718

Purpose

Lentiviral vector expressing a miR-146a decoy sponge

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

14X miR-146a binding sites

Use

Lentiviral

Expression

Mammalian

Mutation

G to T mutation in position 75 of the insert

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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p7128 pHAGE-P-CMVt-N-HA-GAW-HLA-A

Plasmid

#100154

Purpose

Expresses HLA-A

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HLA-A (HLA-A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTH-PanCen TALE-mVenusN

Plasmid

#191395

Purpose

TALE fused to N-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

N-terminal part of split mVenus

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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MTK3_026

Plasmid

#123741

Purpose

Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Flamindo2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCAT.011

Plasmid

#119555

Purpose

Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQE60 Trx1 SSAAA

Plasmid

#98403

Purpose

Bacterial expression vector for Trx1 SSAAA

Depositor

Tobias Dick

Article

Schwertassek et al EMBO J. 2007 Jul 11;26(13):3086-97. Epub

Insert

Trx1 SSAAA (TXN Human)

Expression

Bacterial

Mutation

C32S, C35S, C62A, C69A, C73A

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pC0.168

Plasmid

#119635

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS2 Down Flank

Use

Synthetic Biology

Mutation

bp 630 A to T

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pKD282

Plasmid

#125072

Purpose

Expression of SO_4427 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4427 (SO_4427 )
lacI (lacI )

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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BB3aZ_14*

Plasmid

#98542

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCA0.002

Plasmid

#119560

Purpose

Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2 Flag Smad3 T179V

Plasmid

#26997

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T179V

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
human K8

Plasmid

#14742

Depositor

M Bishr Omary

Article

Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

Insert

Keratin 8 (KRT8 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
SERA1-bio-His

Plasmid

#50812

Purpose

Expresses enzymatically monobiotinylated full length SERA1

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

SERA1

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST(frt)(ATG_V5-APEX2_noSTOP)

Plasmid

#168207

Purpose

Destination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selection

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Type

Empty backbone

Use

Unspecified; Pdest gateway

Tags

V5 APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_GART

Plasmid

#183296

Purpose

All-in-One CRISPRko system with a guide RNA that targets GART gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

GART

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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