19,660 results

1. pACYC-T7

   Plasmid

   #41187

   Depositor

   Dan Bolon

   Article

   Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

   Insert

   S. cerevisiae Hsp82
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSKI015

   Plasmid

   #11570

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Expression

   Plant

   Available Since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-CAG-empty-pgk-hph

   Plasmid

   #48753

   Purpose

   PiggyBac backbone, CAG promoter, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Type

   Empty backbone

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

4. mEmerald-CD9-10

   Plasmid

   #54029

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CD9 (CD9 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pVQ CMV NanoV1-2a-EGFP ferritin

   Plasmid

   #79649

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanillin 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-HK2

   Plasmid

   #23854

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   HK2 (HK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. ∆DUSP6

   Plasmid

   #27977

   Depositor

   Igor Astsaturov

   Article

   Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

   Insert

   DUSP6 (DUSP6 Human)
   

   Use

   Lentiviral

   Tags

   HA and MYC

   Expression

   Mammalian

   Mutation

   C293S: mutation in the phosphatase domain

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pSBbi-Neo

   Plasmid

   #60525

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-BLM

   Plasmid

   #110299

   Purpose

   Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

   Depositor

   Chris Kok-Lung Chan

   Insert

   BLM (Bloom's Syndrome) helicase (BLM Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. TNFa-GFP

    Plasmid

    #163660

    Purpose

    Expresses GFP tagged TNFa in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    tumor necrosis factor (TNF Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATGL*-V5-APEX2

    Plasmid

    #170574

    Purpose

    A C-terminal APEX2 fusion to the human ATGL S47A protein

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    ATGL* (PNPLA2 Human)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    changed serine 47 to alanine

    Promoter

    CMV

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pet28a_Synthetic_Human_H2A.1

    Plasmid

    #42634

    Depositor

    Joe Landry

    Insert

    Human H2A.1 (H2AC13 Human, Synthetic)
    

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Codon Optimized

    Promoter

    T7

    Available Since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY109 (lenti-LbCpf1)

    Plasmid

    #84740

    Purpose

    Lenti virus delivery of LbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    3xHA, NLS, and P2A

    Promoter

    EFS

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe EGFR Del1

    Plasmid

    #32062

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Del1(E746-A750)

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDESTmycDICER

    Plasmid

    #19873

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    dicer 1, ribonuclease type III (DICER1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH0001

    Plasmid

    #85969

    Purpose

    UCOE-SFFV-dCas9-BFP-KRAB

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BFP-KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    SFFV

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP(C1)-PP1alpha

    Plasmid

    #44224

    Depositor

    Angus Lamond, Laura Trinkle-Mulcahy

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1A (PPP1CA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBAD-pHuji

    Plasmid

    #61555

    Purpose

    Red fluorescent protein based pH indicator in bacterial expression vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

20. ampkar

    Plasmid

    #35097

    Purpose

    A fluorescent reporter of AMPK activity and cellular energy stress

    Depositor

    Lewis Cantley

    Article

    Tsou et al Cell Metab. 2011 Apr 6;13(4):476-86.

    Insert

    ampkar
    

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    na

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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