We narrowed to 6,078 results for: nls

1. pDONR207-Citrine-NLS

   Plasmid

   #44586

   Purpose

   Gateway entry vector for Citrine fluorescent protein with a nuclear localization signal

   Depositor

   Yutaka Kodama

   Article

   Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

   Insert

   Citrine-NLS
   

   Use

   Tags

   NLS (Nuclear Localization Signal)

   Expression

   Mutation

   Promoter

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CD8a-cNLS-EGFP

   Plasmid

   #86052

   Purpose

   A mammalian expression plasmid expressing CD8a luminal and transmembrane domains followed by cNLS (classical nuclear localization signal) and C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CD8a luminal and transmembrane along with two SV4…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Dendra2-PCNA-19-SV40NLS-4

   Plasmid

   #57735

   Purpose

   Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. mTFP1-PCNA-19-SV40NLS-4

   Plasmid

   #55505

   Purpose

   Localization: Nucleus, Excitation: 462, Emission: 492

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. GFP-Drosha270-1374delNLS2

   Plasmid

   #62532

   Purpose

   N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   truncated human Drosha mutant (DROSHA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-269 and nuclear localizatio…

   Promoter

   Available since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

6. mKikGR-PCNA-19-SV40NLS-4

   Plasmid

   #57328

   Purpose

   Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mKikGR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. 1xNLS-pMJ915v2-sfGFP

   Plasmid

   #88919

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mKO2-PCNA-19-SV40NLS-4

   Plasmid

   #57890

   Purpose

   Localization: Nucleus, Excitation: 551, Emission: 565

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mKO2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. 2xNLS-pMJ915v2-sfGFP

   Plasmid

   #88920

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-BE3-FNLS

    Plasmid

    #110836

    Purpose

    CMV expression vector for BE3-FNLS construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-FNLS
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    NLS sequence at the N-terminus

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEBG-TFII-deltaNLS1

    Plasmid

    #22153

    Depositor

    Ananda Roy

    Article

    Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    deletion of amino acids 297-304

    Promoter

    Available since

    Nov. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mEmerald-PCNA-19-SV40NLS-4

    Plasmid

    #54223

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    3xSV40NLS and mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. mOrange2-PCNA-19-SV40NLS-4

    Plasmid

    #57971

    Purpose

    Localization: Nucleus, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-2NLS-Cas9

    Plasmid

    #229773

    Purpose

    Expresses Cas9 driven by a CMV promoter

    Depositor

    Yujia Cai

    Article

    Ling et al Nat Nanotechnol. 2025 Apr;20(4):543-553.

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-RfxCas13d-2C-NLS

    Plasmid

    #239816

    Purpose

    Plasmid to carry out IVT of RfxCas13d with SV40 and nucleoplasmin long-NLP NLS (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos

    Article

    Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    Nucleoplasmin long NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTcTurboID-GW_GFP-NLS

    Plasmid

    #234716

    Purpose

    Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.

    Depositor

    Esteban Serra

    Insert

    GFP-3xNLS
    

    Use

    Trypanosomatid expression

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    T7

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-TDP-43 (PY-NLS)

    Plasmid

    #235490

    Purpose

    Expression of chimeric human TDP-43-EGFP bearing a PY-nuclear localization sequence (NLS) from HNRNPA1 in place of the native NLS sequence

    Depositor

    Maxime Rousseaux

    Article

    Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

    Insert

    TARDBP (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Replaced native NLS with PY-NLS from HNRNPA

    Promoter

    Available since

    May 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. UAST-uMCas12a+nls2x

    Plasmid

    #230909

    Purpose

    inducible expression of Cas12a+ with Gal4.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV_dCas9-AsLOV2x3-NLSx3

    Plasmid

    #231567

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    dCas9-AsLOV2x3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAC3-C-GFP-NLS

    Plasmid

    #222604

    Purpose

    Expression of MAC3-tagged GFP-NLS (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 6, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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