165,343 results

1. dCas12j3 VPR

   Plasmid

   #188517

   Purpose

   Expresses FLAG tagged dCas12j3 VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12j3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D413A

   Promoter

   EF1a

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRK246

   Plasmid

   #173741

   Purpose

   donor plasmid for SapTrap assembly

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   wrt-2 promoter
   

   Use

   Other

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD373

   Plasmid

   #137021

   Purpose

   SapTrap GFP-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized GFP
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MS2-epegRNA-Dnmt1

   Plasmid

   #211372

   Purpose

   Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MS2-epegRNA
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMLS714

   Plasmid

   #188886

   Purpose

   MiniMos Transposon vector carrying Pmex-5::2xNLS::Cas9 and floxed Cbr-Unc-119

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

   Insert

   Pmex-5::Cas9
   

   Expression

   Worm

   Mutation

   C. elegans codom optimization and intron addition

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.3_OCT4

   Plasmid

   #26816

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-OCT4-3'UTR (POU5F1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pHS2 (hANGPTL4-V5)

   Plasmid

   #102446

   Purpose

   Expresses V5-tagged human ANGPTL4 in mammalian cells

   Depositor

   Brandon Davies

   Article

   Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

   Insert

   ANGPTL4 (ANGPTL4 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.GFAP.Cre.WPRE.hGH

   Plasmid

   #105550

   Purpose

   AAV expression of Cre from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB and AAV5

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmScarlet_peroxisome_C1

   Plasmid

   #85063

   Purpose

   In vivo visualization of the peroxisome (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Peroxisomal targeting sequence
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. dg264_pENTR_ChronosCel

    Plasmid

    #66102

    Purpose

    Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chronos
    

    Use

    Gateway

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid

    #160437

    Purpose

    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination

    Depositor

    Nicholas Tolwinski

    Article

    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi

    Insert

    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    

    Tags

    Cleavage signal

    Expression

    Bacterial

    Mutation

    Codon-optimized for expression in C. elegans

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)

    Plasmid

    #174529

    Purpose

    Dual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiency

    Depositor

    Yu-Hsuan Tsai

    Article

    Mills et al Cell Rep Methods. 2021 Sep 16;1(6):10007

    Inserts

    PylS
    PylT
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11b-MIF

    Plasmid

    #149446

    Purpose

    Expression of WT MIF in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCVpuro-PCAF

    Plasmid

    #63705

    Purpose

    retroviral expression of human PCAF in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    PCAF (KAT2B Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    E452G

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162379

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE-mCherry-p2A-eNpHR3.0-TS

    Plasmid

    #158703

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-eNpHR3.0-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-F in pAR-9

    Plasmid

    #166921

    Purpose

    AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F

    Depositor

    Casey Maguire

    Article

    Hanlon et al Mol Ther Methods Clin Dev. 2019 Oct 23;1

    Insert

    REP/CAP
    

    Use

    AAV

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SPHK1 gRNA (BRDN0001147534)

    Plasmid

    #76814

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SPHK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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