175,318 results

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pCMV-HA-hIFITM2

Plasmid

#58398

Purpose

Expressed human IFITM2 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM2 (IFITM2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pL-CRISPR.EFS.PAC

Plasmid

#57828

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Exo84

Plasmid

#53762

Purpose

pEGFP-C3 plasmid that expresses Exo84

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo84 (EXOC8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

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mAzurite-N1

Plasmid

#54617

Purpose

Localization: N1 Cloning Vector, Excitation: 384, Emission: 450

Depositor

Patrick Daugherty, Michael Davidson

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Type

Empty backbone

Tags

mAzurite

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mT-Sapphire-N1

Plasmid

#54569

Purpose

Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Type

Empty backbone

Tags

mT

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Trx-mSA2

Plasmid

#52320

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Article

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, S tag, TEV site, and Thioredoxin

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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IFP2.0-N1

Plasmid

#54785

Purpose

Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Type

Empty backbone

Tags

IFP2.0

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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DsRed2-C1

Plasmid

#54611

Purpose

Localization: C1 Cloning Vector, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

DsRed2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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HO-Poly-KanMX4-HO

Plasmid

#51662

Purpose

yeast plasmid for integration of target sequence at HO locus, Kan selection

Depositor

David Stillman

Article

Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

Type

Empty backbone

Use

Yeast integration

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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ePop

Plasmid

#50953

Purpose

Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

Depositor

Lingchong You

Article

Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

Inserts

E lysis protein
LuxI
LuxR

Mutation

Early stop codon introduced in mutated luxR resul…

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-T2A-mCherry

Plasmid

#50972

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Has Service

AAV8

Insert

Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)

Use

AAV

Tags

T2A-mCherry and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCFD2-dU6:2gRNA

Plasmid

#49409

Purpose

expression of gRNA under control of the Drosophila U6:2 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-2:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-2

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pRK5

Plasmid

#46333

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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FNpCDNA3

Plasmid

#45346

Depositor

Robert Oshima

Type

Empty backbone

Tags

FLAG and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX-NICD

Plasmid

#44471

Depositor

Ernesto Canalis

Article

Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

Insert

Notch intracellular domain (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains amino acid 1753 to C-Term

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only
pBabepuroBAF180

Plasmid

#41078

Depositor

Ramon Parsons

Article

Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

Insert

BAF180 (PBRM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

3 silent mutations: 3595 a to g; 4981 a to t; 587…

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCXLE-hGLIS1

Plasmid

#37625

Purpose

Non-integrating (episomal) expression of human GLIS1

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

human GLIS1 (GLIS1 Human)

Expression

Mammalian

Mutation

T110A, A187G, I221T

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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