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DNMT3L-dCas9

Plasmid

#126587

Purpose

DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

DNMT3L (DNMT3L Human)

Use

CRISPR

Tags

3XFLag-NLS-DNMT3L-dCas9-NLS

Expression

Mammalian

Available Since

Dec. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER12 (miR-RUL)

Plasmid

#46935

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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p168-759

Plasmid

#62619

Purpose

ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLL7.0: mTiam1(64-437)-tgRFPt-SSPB R73Q

Plasmid

#60418

Purpose

Mammalian Expression of Tiam DH/PH-tgRFPt-Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

mTiam1-tgRFPt-SSPB R73Q

Use

Cre/Lox and Lentiviral

Tags

tgRFPt

Expression

Mammalian

Mutation

mTiam 64-437 // SSPB R73Q, Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-hIes2

Plasmid

#15355

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

PAP-1 binding protein (INO80B Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
HSP70l-APEX2-GBP in pDEST-Tol2-pA2-acrys-mCherry

Plasmid

#71282

Purpose

Zebrafish expression vector for heat-shock inducible expression of APEX2-GBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Use

Zebrafish expression with tol2

Promoter

HSP70l

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENN491_TALEcharm_MmT8x

Plasmid

#220836

Purpose

TALEcharm Kv2 expression in mammalian cells; targets Prnp

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGM190

Plasmid

#69614

Purpose

inducible gene expression in Streptomyces

Depositor

Günther Muth

Article

Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

Inserts

aphII
tsr
rep
sso
TipA promoter
dso

Use

Streptomyces- e. coli shuttle vector

Expression

Bacterial

Available Since

Jan. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE CACNG8-GFP KI #2

Plasmid

#131474

Purpose

Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: A408)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cacng8 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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2PH-PLCd-APEX2-P2A-mKate2

Plasmid

#71284

Purpose

Mammalian expression vector driving 2PH-PLC-delta-APEX2 (Human 2xPLCd PH domain, [marks PI(4,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2PH-PLCd-APEX2-P2A-mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCh-Nck

Plasmid

#60034

Purpose

Expresses a fusion containing CRY2olig (PHR, E490G) with mCherry and 3 SH3 domains of Nck

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2-mCh-NCK (CRY2 Human, Mustard Weed)

Tags

HA tag

Expression

Mammalian

Mutation

M354I, E490G CRY2

Promoter

CMV

Available Since

Nov. 12, 2014

Availability

Academic Institutions and Nonprofits only

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VZV ORF 60 (gL)

Plasmid

#64082

Purpose

eukaryotic expression plasmid for VZV glycoprotein L

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein L

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pSW646 - pML1>>

Plasmid

#115995

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-CreLite

Plasmid

#131783

Purpose

Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC, PIF6CreN, and mTagBFP2

Use

Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

Tags

mTagBFP2

Expression

Mammalian

Promoter

Ubi

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF45-2xCSTREP

Plasmid

#136206

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF45

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF45

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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VZV ORF 37(gH)

Plasmid

#64081

Purpose

eukaryotic expression plasmid for VZV glycoprotein H

Depositor

Randall Cohrs

Article

Birlea et al J Virol. 2013 Jan;87(1):415-21. doi: 10.

Insert

VZV glycoprotein H

Expression

Mammalian

Promoter

CMV IE with intron

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
mRuby2-Zyxin-6

Plasmid

#55917

Purpose

Localization: Focal Adhesions, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Zyxin (ZYX Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pORANGE Shank1-GFP KI

Plasmid

#131500

Purpose

Endogenous tagging of Shank1: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Shank1, Shank1 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-tTA

Plasmid

#127091

Purpose

An AAV genome with Cre-dependent expression of tTA from the CAG promoter

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+-PIF6-EGFP

Plasmid

#154913

Purpose

1-100 amino acids of Arabidopsis PIF6 (Gene ID: 825382), tagged with EGFP fluorescent protein. Mammalian codon optimised.

Depositor

Clare Buckley, Jonathan Clarke

Article

Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

Insert

phytochrome interacting factor 3-like 2 (PIL2 Mustard Weed)

Use

Xenopus/avian/zebrafish

Tags

EGFP

Expression

Mammalian

Mutation

1-100 amino acids of Arabidopsis PIF6

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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