159,132 results

1. pRPF215

   Plasmid

   #106377

   Purpose

   C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

   Depositor

   Robert Fagan, Neil Fairweather

   Article

   Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

   Insert

   Ptet-himar1-Ter(slpA)-transposon
   

   Use

   E. coli - c. difficile shuttle vector

   Tags

   Expression

   Mutation

   Codon optimised for C. difficile

   Promoter

   Ptet

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

   Plasmid

   #234952

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TLCV2-Scal-T2A-EGFP-Puro

   Plasmid

   #234949

   Purpose

   Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-ApaLI-T2A-EGFP-Puro

   Plasmid

   #234948

   Purpose

   Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAdDeltaF6

   Plasmid

   #112867

   Purpose

   AAV helper plasmid

   Depositor

   James M. Wilson

   Insert

   E4, E2a and VA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   David Schaffer, John Flannery

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   7mer insertion in the AAV2 cap

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIV-D3psi/delta env/delta Vif/delta Vpr

   Plasmid

   #132928

   Purpose

   Production of SIV-VLPs containing Vpx protein

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   Gag, pol, and vpx
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-3XHA-EGFP-OMP25

   Plasmid

   #83356

   Purpose

   For tagging mitochondria with HA epitopes (HA-MITO construct)

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XHA-EGFP-OMP25
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCmini-iCAP-AAV1.X1

    Plasmid

    #209780

    Purpose

    non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

    Insert

    Synthetic construct isolate AAV1-X1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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