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Plasmid#108867PurposeExpresses human Tau with a mutated cysteineDepositorInsertTau (MAPT Human)
UseTagsExpressionBacterialMutationI260C C291A C322A to allow maleimide dye labellingPromoterT7Available sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V-NC-MS2
Plasmid#122944PurposeMS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particlesDepositorInsertNC-MCP (MS2 coat protein) fusion protein in Gag
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
psPAX2-D64V-NC-PP7
Plasmid#122945PurposePP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particlesDepositorInsertNC-PCP (PP7 coat protein) fusion protein in Gag
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDLg/pRRE
Plasmid#12251Purpose3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)DepositorInsertHIV-1 GAG/POL
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8001 - pCMV-R8.91 (D64V)
Plasmid#202687PurposeR8.91 plasmid with mutated integrase (D64V)DepositorInsertgag pol
UseLentiviralTagsExpressionMammalianMutationD64VPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 EGFP-Tau
Plasmid#108868PurposeExpresses EGFP linked to human TauDepositorInsertTau (MAPT Human)
UseLentiviralTagsEGFPExpressionMammalianMutationhuman four-repeat tau lacking the N-terminal sequ…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJJH1320
Plasmid#36915DepositorInsertCre recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSV-Rev
Plasmid#12253Purpose3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)DepositorInsertRev (rev )
UseLentiviral; PackagingTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUMVC
Plasmid#8449PurposePackaging plasmid for producing MuLV retroviral particles. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RFP
Plasmid#22910DepositorInsertChicken Beta-actin promoter driving DsRedExpress
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM
Plasmid#12247Purpose2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
HRE:GFP-pGK:Cre
Plasmid#68552PurposeLentivirus expressing hypoxia-responsive GFP reporter and CreDepositorInsertsGreen Fluorescent Protein
Cre
UseLentiviralTagsExpressionMammalianMutationPromoterHRE and pGKAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB22.SV40.Zeo
Plasmid#135692PurposeLentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selectionDepositorInsertmammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-RFP
Plasmid#22907DepositorInserthuman Synapsin I promoter driving DsRedExpress (SYN1 Human)
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH*deltavpu/RT-
Plasmid#136985PurposeSecond-generation lentiviral packaging plasmid lacking reverse transcriptase activityDepositorInsertHIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
UseTagsExpressionMammalianMutationcarries D110E mutation in the reverse transcripta…PromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
nuc-mCherry-pTRIP
Plasmid#163520PurposeUsed to construct lentivirus to express nucleus-targeted mCherry in mammalian cellsDepositorInsertnucleus targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
mem-mCherry-pTRIP
Plasmid#163521PurposeUsed to construct lentivirus to express membrane-targeted mCherry in mammalian cellsDepositorInsertmembrane targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
katushka-FMDV-P2A-pTRIP
Plasmid#163522PurposeUsed to construct lentivirus to express katushka in mammalian cells with option for a second insertDepositorInsertstopless Katushka-P2A
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mCAMK-RFP
Plasmid#22908DepositorInsertmouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
Plasmid#51656Purposehsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrangeDepositorInsertQuasar2-mOrange-Chr64-GFP
UseLentiviralTagsGFP and mOrangeExpressionMammalianMutationPromoterhuman synapsinAvailable sinceMarch 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector
Plasmid#132560PurposeVector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.DepositorInsertsABEmax
sgRNA, with Tetraloop replaced by com aptamer
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-ABE-3'UTR-sgRNA-2xMS2
Plasmid#132554PurposeVector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2DepositorInsertsABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
WT-MEK1-V5
Plasmid#217297PurposeLentivirus vector for expression of MEK1DepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MEK1-K57N-V5
Plasmid#217300PurposeLentivirus vector for expression of MEK1 K57NDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationK57NPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
MEK1-Q56P-V5
Plasmid#217299PurposeLentivirus vector for expression of MEK1 Q56PDepositorInsertMAP2K1 (MAP2K1 Human)
UseLentiviralTagsV5ExpressionMutationQ56PPromoterAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-Pol2-ffLuc2-eGFP
Plasmid#71394PurposeLentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoterDepositorInsertPol2-ffLuc2-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterPol2 (mouse RNA polymerase II gene promtoer)Available sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GExpressionMutationS34C mutation in NefPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FerH-ffLuc2-eGFP
Plasmid#71393PurposeLentiviral vector of luciferase-eGFP fusion gene driven by FerH promoterDepositorInsertFerH-ffLuc2-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterFerHAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-1xPP7-2x3'UTR
Plasmid#122947PurposeFor expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTRDepositorInsertSaCas9-PP7-HBB 3' UTR
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only