4,133 results

1. Tet-pLKO-puro

   Plasmid

   #21915

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmGFP-ADAR1-p110

   Plasmid

   #117928

   Purpose

   ADAR1-p110 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR1-p110 (ADAR Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP- SEC61B

   Plasmid

   #121159

   Purpose

   Mammalian expression of GFP tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_EGFP

   Plasmid

   #25899

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmGFP-ADAR2

   Plasmid

   #117929

   Purpose

   ADAR2 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR2 (ADARB1 Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti.PGK.blast-Renilla_Luciferase

   Plasmid

   #74444

   Purpose

   Control lentiviral vector expressing Renilla luciferase

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Renilla Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-CMV-GFP

   Plasmid

   #67634

   Purpose

   AAV vector expressing GFP

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmEGFP-C1

   Plasmid

   #36412

   Depositor

   Benjamin Glick

   Type

   Empty backbone

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-TLR5-CFP

   Plasmid

   #13019

   Depositor

   Doug Golenbock

   Insert

   Toll-Like Receptor 5 (TLR5 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-scFvGCN4sfGFPTET1CD

    Plasmid

    #82561

    Purpose

    Expression vector for antibody-sfGFP-TET1CD.

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    scFvGCN4sfGFPTET1CD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Promoter

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIS0

    Plasmid

    #12178

    Purpose

    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

    Depositor

    David Bartel

    Article

    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.2 TDP-43 YFP

    Plasmid

    #84911

    Purpose

    expression of TDP-43 YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-HIF2alpha-P405A/P531A-pcDNA3

    Plasmid

    #18956

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-EGFP-Rac1-Q61L

    Plasmid

    #12981

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-dCas9-5xPlat2AflD

    Plasmid

    #82560

    Purpose

    Expression vector for Cas9 peptide array (linker length: 22aa).

    Depositor

    Izuho Hatada

    Article

    Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

    Insert

    dCas9-5xPlat2AflD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3xFLAG-CMV10-mApg16L

    Plasmid

    #24302

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

    Insert

    autophagy related genes 16L (Atg16l1 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    V583M

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHA-eIF4E

    Plasmid

    #17343

    Depositor

    Dong-Er Zhang

    Article

    Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

    Insert

    eIF4E (EIF4E Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-TLR2-CFP

    Plasmid

    #13015

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 2 (TLR2 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBMN FKBP(DD)-YFP

    Plasmid

    #31763

    Depositor

    Thomas Wandless

    Article

    Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

    Insert

    FKBP(DD)-YFP-HA (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    F36V, L106P on FKBP12

    Promoter

    LTR

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV4-Tax WT

    Plasmid

    #23284

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    Tax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HA-AMOT p130

    Plasmid

    #32821

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

    Insert

    AMOT p130 (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    E5D

    Promoter

    CMV

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3 HA eIF4GI (1-1599)

    Plasmid

    #45640

    Depositor

    Nahum Sonenberg

    Article

    Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

    Insert

    eIF4GI transcript variant 2 (EIF4G1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFlag-CMV1-hMD2

    Plasmid

    #13028

    Depositor

    Doug Golenbock

    Insert

    MD2 (LY96 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-HA2-ROC1

    Plasmid

    #19897

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCDNA3-myc3-ROC1

    Plasmid

    #20717

    Depositor

    Yue Xiong

    Article

    He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

    Insert

    RING finger protein 1 (ROC1) (RBX1 Human)
    

    Use

    Tags

    3xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-EGFP-RhoA-T19N

    Plasmid

    #12967

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    RhoA DN (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T19N Dominant negative

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 c-SRC (WT)

    Plasmid

    #42202

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    c-SRC (SRC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L176F mutation compared to reference sequence NM_…

    Promoter

    CMV

    Available since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge