We narrowed to 2,111 results for: D10

1. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

2. AV44_pCAG.Cas9-D10A.gRNA.S1

   Plasmid

   #199258

   Purpose

   Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9-D10A nickase
   AAVS1-targeting gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-MFSD10_STOP

   Plasmid

   #161056

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   MFSD10 (MFSD10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A_D10A-1x4

   Plasmid

   #58774

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330A_D10A-1x6

   Plasmid

   #58776

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. wt HA-dynamin 1 pUHD10-3

   Plasmid

   #34688

   Depositor

   Sandra Schmid

   Article

   Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

   Insert

   dynamin 1 (DNM1L Human)
   

   Use

   Tags

   HA (variant)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

8. HOXD10-pCDNA

   Plasmid

   #21007

   Depositor

   Corey Largman

   Insert

   HOXD10 (HOXD10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pAtCas9-D10A_2

   Plasmid

   #91160

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A (nickase)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6nH1-HD10kb

    Plasmid

    #172657

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 10204-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD10kbA/pegRNA-HD10kbB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. HOXD10-pSG5

    Plasmid

    #21008

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    HOXD10 (HOXD10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGs-Hoxd10

    Plasmid

    #45721

    Depositor

    Jeremy Dasen

    Article

    Dasen et al Cell. 2008 Jul 25;134(2):304-16. doi: 10

    Insert

    Hoxd10 (HOXD10 Chicken)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CMV / b-actin

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pX330A_D10A-1x3

    Plasmid

    #58773

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTaCas9-D10A_5

    Plasmid

    #91170

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAD108_Arg273His_pCMV6Entry-hIRG1

    Plasmid

    #124878

    Purpose

    Expresses hCAD Arg273His mutant in mammalian cells

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    myc and FLAG tags

    Expression

    Mammalian

    Mutation

    mutated Arg273 to His

    Promoter

    CMV

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRPB1-CTD10

    Plasmid

    #112821

    Purpose

    pSMF2 with 10 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTaCas9-D10A_6

    Plasmid

    #91171

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAtCas9-D10A_3

    Plasmid

    #91161

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Hoxd10 probe

    Plasmid

    #34712

    Depositor

    Ellen Carpenter

    Article

    Choe et al Gene Expr Patterns. 2006 Oct;6(7):730-7.

    Insert

    Hoxd10 (Hoxd10 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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