We narrowed to 5,784 results for: pcas

1. pCAG_smFP HA

   Plasmid

   #59759

   Purpose

   encodes multiple copies of an epitope tag HA

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _HA
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG1.1

   Plasmid

   #173685

   Purpose

   The expression vector for genes of interest under CAG promoter.

   Depositor

   Masahito Ikawa

   Article

   Ikawa et al J Biol Chem. 2011 Feb 18;286(7):5639-46.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_smFP V5

   Plasmid

   #59758

   Purpose

   encodes multiple copies of an epitope tag V5

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Insert

   smFP _V5
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS_3E5E_eGFP

   Plasmid

   #103054

   Purpose

   Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebola virus (ebolavirus Zaire, Mayinga) minigenome
   

   Tags

   3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAH01

   Plasmid

   #128162

   Purpose

   IncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteria

   Depositor

   Calvin Henard, Michael Guarnieri

   Article

   Henard et al Sci Rep. 2016 Feb 23;6:21585. doi: 10.10

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS_3E5E_luciferase

   Plasmid

   #103055

   Purpose

   Encodes Ebola virus minigenome with firefly luciferase reporter gene. Minigenome is under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   Ebolavirus minigenome
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGIG

   Plasmid

   #11159

   Purpose

   Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Expression

   Mammalian

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS_cAMPFIRE-M

   Plasmid

   #182284

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-M (cAMP sensor)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT001

   Plasmid

   #171061

   Purpose

   Plasmid for expressing HIV-1 envelope glycoprotein

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   HIV-1 Env
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Expression

    Bacterial

    Promoter

    prab17

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAMBgfp

    Plasmid

    #206130

    Purpose

    This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

    Depositor

    Anne Osbourn

    Article

    Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

    Insert

    hygromycin resistance gene and the Promoter of ToxA+SGFP
    

    Use

    Binary vector for expression of sgfp in filamento…

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG_iPE-N

    Plasmid

    #214734

    Purpose

    encodes iPE-N prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the N terminus of nCas9-H840A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-N_bGH
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_iPE-C

    Plasmid

    #214735

    Purpose

    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    CAG_iPE-C_bGH
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-oG

    Plasmid

    #74288

    Purpose

    To express oG (optimized Glycoprotein) under CAG promoter

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG
    

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_cAMPFIRE-L

    Plasmid

    #182283

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CAG promotor w/ WPRE translation enhancer.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAT203

    Plasmid

    #134880

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCACTUS

    Plasmid

    #167559

    Purpose

    Suicide vector with the sacB gene (as a contraselective marker), CmlR gene, and temperature sensitive ori, used to perform allelic exchange mutagenesis.

    Depositor

    Renato Morona

    Article

    Morona et al J Bacteriol. 1995 Feb;177(4):1059-68. do

    Type

    Empty backbone

    Use

    Suicide cloning vector, temperature sensitive.

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAT009

    Plasmid

    #171635

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting B2M and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG_eGCaMP(2+)

    Plasmid

    #201148

    Purpose

    expresses eGCaMP2+ in mammalian cells

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP2+
    

    Expression

    Mammalian

    Available Since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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