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Showing: 1951 - 2000 of 22679 results

  1. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid
    #225944
    Purpose
    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Evan Reid, Bart Nieuwenhuis
    Inserts
    ATL1 (ATL1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225944-plasmid-map-sequence-id-446838

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  2. pETcon-pGAL1-Aga2-Amyloid β42-c_myc

    Plasmid
    #225223
    Purpose
    Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360
    Inserts
    Amyloid β42
    Aga2 (AGA2 Budding Yeast)
    Use
    Tags
    HA Tag and c-myc Tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225223-plasmid-map-sequence-id-460494

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  44. pCA528 IST1 L328D (316-366)(C-Cys)

    Plasmid
    #193035
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L328D, Residues 316-366, Non-native C-terminal Cy…
    Promoter
    T7
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193035-plasmid-map-sequence-id-379007

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  45. pCA528 IST1 L355A (316-366)(C-Cys)

    Plasmid
    #193036
    Purpose
    Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    IST1 (IST1 Human)
    Use
    Tags
    6xHis-Sumo
    Expression
    Bacterial
    Mutation
    L355A, Residues 316-366, Non-native C-terminal Cy…
    Promoter
    T7
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193036-plasmid-map-sequence-id-379013

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  48. pUAS-Myc-BirA-G3-ER

    Plasmid
    #173815
    Purpose
    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site
    Depositor
    Norbert Perrimon
    Article
    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:
    Insert
    Myc-BirA-G3-ER
    Use
    Tags
    Myc
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    173815-plasmid-map-sequence-id-340316

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  49. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid
    #217768
    Purpose
    Fluorescent reporter for ceramide (for stable mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Sleeping beauty transposase-compatible genome ins…
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    EF-1alpha promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217768-plasmid-map-sequence-id-435794

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  50. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid
    #217766
    Purpose
    Fluorescent reporter for ceramide (for bacterial expression and purification)
    Depositor
    Paula Nunes-Hasler
    Article
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400 plus vector-based linker LE…
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217766-plasmid-map-sequence-id-435787

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Showing: 1951 - 2000 of 22679 results