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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T
Plasmid#225062PurposeExpression and Nuclear-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM
Plasmid#225061PurposeExpression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cellsDepositorInsertCylLL-S15T
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationS15TPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUiLMO2W
Plasmid#129480Purpose3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin CAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-iLMO2
Plasmid#129474PurposeMammalian expression vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CapChR1_pUAST-attb
Plasmid#188040PurposeExpresses CapChR1 in DrosophilaDepositorInsertCapChR1
UseTagsnoneExpressionInsectMutationS63D, L132C, T159C and N258E mutations in CrChR2PromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa
Plasmid#127389PurposeFluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alphaDepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterpCAGAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV BiP-Myc-KDEL-wt
Plasmid#27164PurposeMammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequenceDepositorInsertBiP (HSPA5 Human)
UseTagsKDEL and MycExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP
Plasmid#194681PurposeCMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_01-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synapto-pHluorin
Plasmid#168510PurposeExpression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.DepositorInsertSynaptophysin-pHluorin (Syp Mouse)
UseTagspHluorinExpressionMammalianMutationPromoterCAGAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP
Plasmid#194683PurposeCMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_05-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_05
Plasmid#194666PurposeProtein production of the split HaloTag based calcium recorder Caprola_05 in bacteriaDepositorInsertCaprola_05
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_eYFP
Plasmid#188035PurposeExpresses CapChR2-eYFP in mammalian neuronsDepositorInsertCapChR2-eYFP
UseAAVTagseYFPExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV gfaABC1D NES-jRCaMP1a
Plasmid#171120PurposeAstrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.DepositorInsertjRCaMP1a
UseAAVTagsHis Tag, T7 Tag, XpressTagExpressionMammalianMutationPromotergfaABC1DAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP
Plasmid#194696PurposeHuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_01-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Caprola_06-mEGFP
Plasmid#194693PurposeCMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transductionDepositorInsertCaprola_06-mEGFP
UseLentiviralTagsmEGFPExpressionMutationPromoterCMVAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Caprola_off-mEGFP
Plasmid#194695PurposeCMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertCaprola_off-mEGFP
UseLentiviralTagsmEGFPExpressionMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn_CapChR2_mScarlet
Plasmid#188039PurposeExpresses CapChR2-mScarlet in mammalian neuronsDepositorInsertCapChR2-mScarlet
UseAAVTagsmScarletExpressionMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterhSynAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only
