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Showing: 8041 - 8060 of 65925 results

  2. 3294_pETcon-SARS2-RBD_Omicron-BA1

    Plasmid
    #184408
    Purpose
    yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD
    Depositor
    Jesse Bloom
    Publication
    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1
    Insert
    SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    Tags
    HA and c-Myc
    Expression
    Yeast
    Mutation
    G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    184408-plasmid-map-sequence-id-362479

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  3. pJK413

    Plasmid
    #155372
    Purpose
    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)
    Depositor
    Pamela Silver
    Publication
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    155372-plasmid-map-sequence-id-309000

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  14. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

    Plasmid
    #219565
    Purpose
    3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Publication
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    HygroR_2A_H2B-GFP-N_loxP_mScarlet
    Use
    Unspecified
    Promoter
    EF-1a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219565-plasmid-map-sequence-id-438331

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  16. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Publication
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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  18. ER-GA

    Plasmid
    #209871
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1
    Depositor
    Sarah Cohen
    Publication
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddGFP A
    Tags
    Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209871-plasmid-map-sequence-id-415464

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Showing: 8041 - 8060 of 65925 results