19,662 results

1. LRG2.1-BFP

   Plasmid

   #120577

   Purpose

   Lentiviral plasmid to co-express a guide RNA and EBFP.

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hFerritinL

   Plasmid

   #122653

   Purpose

   Expression of hFerritinL in bacterial cells

   Depositor

   Trevor Douglas

   Insert

   light chain ferritin, human (FTL Human)
   

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CBh-DIO-EGFP

   Plasmid

   #87168

   Purpose

   Expression of eGFP in the presence of Cre

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Insert

   EGFP
   

   Use

   AAV

   Tags

   None

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV9:cTNT::3Flag-hYAP S127A

   Plasmid

   #86558

   Purpose

   To produce cardiac specific adeno-associated virus expressing activated human YAP.

   Depositor

   William Pu

   Article

   Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

   Insert

   YAP (YAP1 Human)
   

   Use

   AAV

   Tags

   3Flag

   Mutation

   Serine 127 was mutated into Alanine, to activate …

   Promoter

   cTNT

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Puro_siKD

   Plasmid

   #86695

   Purpose

   Tetracycline inducible expression of shRNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   H1 TO

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE3G-Bi-α-chain

   Plasmid

   #133730

   Purpose

   Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   a-Chain_gePSI
   ZsGreen1
   

   Expression

   Mammalian

   Mutation

   Only one part of the gePSI-system (aa1-141 + stop…

   Promoter

   pTRE3G-Bi

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG32

   Plasmid

   #35122

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   hphMX4
   

   Use

   Yeast replicating

   Available Since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.pU1a-SpCas9

   Plasmid

   #121507

   Purpose

   Expresses codon-optimized SpCas9 in mammalian cells.

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. FURIN-bio-His

    Plasmid

    #51755

    Purpose

    Expresses full-length Furin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    FURIN (FURIN Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and TRE

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDB302

    Plasmid

    #113673

    Purpose

    expresses Cth SUMO protease in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Cth SUMO protease
    

    Tags

    10-His

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-934

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FlagMLL-pl-ENL(5613)

    Plasmid

    #20873

    Depositor

    Robert Slany

    Article

    Bach et al Oncogene. 2009 Feb 12. 28(6):815-23.

    Insert

    MLL (KMT2A, MLLT1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST40-2XFL-wtSrc

    Plasmid

    #140295

    Purpose

    Expresses Flag-tagged wild-type human Src in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src wildtype (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV flag ERR alpha

    Plasmid

    #10975

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

    Insert

    ERR alpha (ESRRA Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Nov. 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHIV_pTp66p51

    Plasmid

    #78233

    Purpose

    Expresses HIV reverse transcriptase

    Depositor

    Christopher Voigt

    Article

    Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

    Inserts

    p66
    p51
    LacI
    

    Expression

    Bacterial

    Promoter

    Const. Promoter (From DNA2.0 Vec) and pTac Minima…

    Available Since

    Aug. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK347 (BSD-P2A-mAID)

    Plasmid

    #121181

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID
    

    Use

    CRISPR

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSNAP-tag (T7) Vector

    Plasmid

    #101137

    Purpose

    Expression of protein of interest with SNAP-Tag in bacterial cells

    Depositor

    New England Biolabs, Ana Egana

    Type

    Empty backbone

    Tags

    SNAP-tag (SNAP26b)

    Expression

    Bacterial

    Available Since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pET-28a-hPKM1

    Plasmid

    #44241

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):181-6. doi

    Insert

    Pyruvate Kinase M1 (PKM Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. CHRM2-Tango

    Plasmid

    #66249

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    CHRM2 (CHRM2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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