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We narrowed to 18,759 results for: nls

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Showing: 1601 - 1640 of 18759 results

  1. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid
    #179586
    Purpose
    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)
    Depositor
    Ali Hemmati Brivanlou
    Publication
    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:
    Insert
    NLS-NG-T2A-hSHH
    Use
    Piggybac
    Tags
    NeonGreen
    Promoter
    TRE
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    179586-plasmid-map-sequence-id-354597

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  6. pDSP23

    Plasmid
    #199372
    Purpose
    Encodes dual cistronic nuclear localized GCaMP6s and mScarlet-I optimized for C. elegans expression
    Depositor
    Hang Lu
    Insert
    NLS::GCaMP6s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    Expression
    Bacterial
    Mutation
    no mutations, except added subcellular localizati…
    Available since
    April 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199372-plasmid-map-sequence-id-390928

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  8. eA3A-BE3 (pRZ206)

    Plasmid
    #131315
    Purpose
    CAG promoter expression plasmid for hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hA3A(N57G)-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131315-plasmid-map-sequence-id-254673

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  13. deltaVP1 only

    Plasmid
    #166675
    Purpose
    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Insert
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166675-plasmid-map-sequence-id-329829

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  14. pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST

    Plasmid
    #109048
    Purpose
    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vector
    Depositor
    Hillel Adesnik
    Publication
    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/
    Has service
    AAV9
    Insert
    eGtACR1-ST
    Use
    AAV
    Promoter
    CAG
    Available since
    July 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109048-plasmid-map-sequence-id-264817

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  15. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid
    #104563
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1 (D832A)
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    104563-plasmid-map-sequence-id-260085

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  16. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131133-plasmid-map-sequence-id-255501

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  17. pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)

    Plasmid
    #185485
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185485-plasmid-map-sequence-id-362670

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  18. pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)

    Plasmid
    #185488
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-P2A-eGFP and NLS
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185488-plasmid-map-sequence-id-362671

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  25. pCMVMagFRET-1-NLS

    Plasmid
    #50748
    Purpose
    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.
    Depositor
    Maarten Merkx
    Publication
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gene encoding for MagFRET-1 with NLS
    Expression
    Mammalian
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50748-plasmid-map-sequence-id-76084

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  27. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid
    #55195
    Purpose
    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    VP64
    Expression
    Mammalian
    Promoter
    CMV/hUBC
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55195-plasmid-map-sequence-id-85582

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  29. xCas9(3.6)-ABE(7.10)

    Plasmid
    #108387
    Purpose
    Mammalian expression vector for xCas9(3.6)-ABE
    Depositor
    David Liu
    Publication
    Hu et al Nature. 2018 Feb 28. pii: nature26155. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    xCas9(3.6)-ABE(7.10)
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …
    Available since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    108387-plasmid-map-sequence-id-207183

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  30. BB3cH_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104907
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104907-plasmid-map-sequence-id-206042

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  36. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  38. NLS-nCas9-NLS-P2A-EGFP (pRZ70)

    Plasmid
    #123616
    Purpose
    CMV promoter expression plasmid for codon-optimized bpNLS-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP (ABEmax control without TadA domains).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-nCas9-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CMV
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123616-plasmid-map-sequence-id-239344

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Showing: 1601 - 1640 of 18759 results