We narrowed to 59,677 results for: plasmid
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Plasmid#63157PurposeDestination vector for Gateway based on vector #394 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter.DepositorTypeEmpty backboneUseCRISPR; Tol2 destination vector for gatewayAvailable sinceApril 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50454PurposeDouble floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorHas serviceAAV8InserthM3D(Gq)-IRES-mCitrine (CHRM3 Human)
UseAAVTagsHAMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPlatTET-gRNA2
Plasmid#82559PurposeAll in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
ExpressionMammalianAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-U6-gRNA-CBh-mCherry
Plasmid#91947PurposeExpression of empty gRNA cassette and mCherryDepositorInsertmCherry
UseAAVAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330A-1x2
Plasmid#58766PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD286 (TagRFP^SEC^3xMyc)
Plasmid#70684PurposeTagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.DepositorInsertTagRFP-T-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xMyc and C. elegans codon-optimized TagRFP-TExpressionWormAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSPgRNA
Plasmid#47108PurposeExpresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cellsDepositorInsertSPgRNA
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3xAP1pGL3 (3xAP-1 in pGL3-basic)
Plasmid#40342DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xAP-1
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains three canonical AP-1 binding sites (TGAC…Available sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV14-3X-Flag-SARS-CoV-2 S
Plasmid#145780PurposeOver expression of SARS-CoV-2 S (spike) in mammalian cell lineDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 S (S SARS CoV 2)
Tags3xFlagExpressionMammalianMutationCodon optimized and C-terminal 19 amino acids del…PromoterCMVAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
ph7SK-gRNA
Plasmid#53189PurposeExpresses the S. pyogenes sgRNA from the 7SK promoterDepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterhuman 7SKAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-eCas9-GFP-U6-gRNA
Plasmid#79145PurposeAll-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in human pluripotent stem cellsDepositorInserteSpCas9(1.1)
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD3-dU6:3gRNA
Plasmid#49410Purposeexpression of gRNA under control of the Drosophila U6:3 promoterDepositorInsertdU6-3:gRNA
UseCRISPRExpressionInsectPromoterdU6-3Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-GFP
Plasmid#32395DepositorPublicationInsertGFP
UseAAV; Adeno-associated virusExpressionMammalianPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-pgk-Cre
Plasmid#24593DepositorHas serviceAAV RetrogradeInsertCre recombinase (codon optimized)
UseAAV and Cre/Lox; Adeno-associatedPromoterpgkAvailable sinceMay 26, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hACE2
Plasmid#1786PurposeMammalian expression of human ACE2DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAngiotensin-converting enzyme 2 (ACE2 Human)
TagsC9 (GTETSQVAPA)ExpressionMammalianAvailable sinceFeb. 14, 2006AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEP4 E02S ET2K
Plasmid#20927Purposeused in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseEpisomal/ebnaAvailable sinceMay 20, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA
Plasmid#154867PurposeFlp-dependent hM4D(Gi) DREADD-mCherryDepositorHas serviceAAV8 and AAV RetrogradeInserthM4D(Gi)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianPromoterHuman Synapsin-1Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV GG hUbC-dsRED
Plasmid#84034PurposeContains Golden Gate cassette to express up to four gRNAs and dsREDDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2
Plasmid#74009PurposeTissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoterDepositorInserts5x UAS
Cas9
T2A
GFP
U6 promoter
UseCRISPRExpressionZebrafishAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-Nanog
Plasmid#13354PurposeRetroviral expression of mouse NanogDepositorAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by