We narrowed to 7,452 results for: Lif

1. pLVX-RT005-HaloGPM

   Plasmid

   #163385

   Purpose

   2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTet_NAD-Snifit-cyto

   Plasmid

   #113914

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNG-Mfn1

   Plasmid

   #195162

   Purpose

   mammalian expression of Mfn1 tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   Mfn1 (MFN1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-Cyto-CaNARi

   Plasmid

   #64849

   Purpose

   Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, Nuclear export signal (NES), and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Nck

   Plasmid

   #186133

   Purpose

   For expression of recombinant Nck in bacteria cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   NCK1 (NCK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acid residues 1-377, with mutations: C139S,…

   Promoter

   Lac

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SB_concave

   Plasmid

   #110102

   Purpose

   pBXPHM3 with scaffold Sybody of the concave library

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Insert

   scaffold Sybody of the concave library
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   May 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEBTet_NADP-Snifit-nucl

   Plasmid

   #113918

   Purpose

   Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEBTet_NAD-Snifit-nucl

   Plasmid

   #113915

   Purpose

   Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NAD-Snifit
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV TetO2

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. N-WASP

   Plasmid

   #186132

   Purpose

   For expression of recombinant N-WASp in bacteria cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   WASL (WASL Rat)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Amino Acid residues 183-193 fused to 273-501

   Promoter

   T7

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTrex-Luc-Neo

    Plasmid

    #48336

    Purpose

    Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

    Depositor

    Rick Tarleton

    Article

    Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

    Insert

    firely luciferase
    

    Use

    Trypanasoma cruzi expression

    Tags

    Expression

    Mutation

    Promoter

    T. cruzi rRNA

    Available since

    July 20, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. AAV.hSyn.GLPLight-ctr

    Plasmid

    #187468

    Purpose

    Expresses GLPLight-ctr in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight-ctr
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    human Synapsin-1

    Available since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SB_loop

    Plasmid

    #110103

    Purpose

    pBXPHM3 with scaffold Sybody of the loop library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the loop library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 12URA-B-ScTop2∆CTD

    Plasmid

    #214924

    Purpose

    Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

    Depositor

    James Berger

    Article

    Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

    Insert

    topoisomerase II
    

    Use

    Tags

    6x His-TEV

    Expression

    Yeast

    Mutation

    Deleted amino acids 1178-1428

    Promoter

    Gal 1/10

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. OSCA1.2

    Plasmid

    #136593

    Purpose

    Expresses the mechanosensitive channel OSCA1.2 in mammalian cells

    Depositor

    Ardem Patapoutian

    Article

    Murthy et al Elife. 2018 Nov 1;7. pii: 41844. doi: 10

    Insert

    OSCA1.2 (AT4G22120 Mustard Weed)
    

    Use

    Tags

    pIRES2-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUE1-TSP-BFLF1

    Plasmid

    #162657

    Purpose

    Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Plasmid

    #205162

    Purpose

    express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-GCN4-HAtag-msfGFP(V206K)-GBI
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. flex-ChrimsonR-EYFP-Kv

    Plasmid

    #135319

    Purpose

    Cre-dependent expression of soma-targeted ChrimsonR

    Depositor

    Hongkui Zeng

    Article

    Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-FKBP-SAC1

    Plasmid

    #108127

    Purpose

    Recruitable SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TagRFP-T-VAPB(1- 218)- eMagB

    Plasmid

    #162255

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    TagRFP-T-VAPB(1- 218)- eMagB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LTR5HS S. pyogenes scaffold CARGO array

    Plasmid

    #191316

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. 3xnls-G-Ca-FLITS

    Plasmid

    #191463

    Purpose

    Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

    Depositor

    Dorus Gadella

    Article

    van der Linden et al bioRxiv 2024.11.05.622032; doi: https://

    Insert

    G-Ca-FLITS
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMA-SpCas9-g2

    Plasmid

    #80785

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SB_convex

    Plasmid

    #110104

    Purpose

    pBXPHM3 with scaffold Sybody of the convex library

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

    Insert

    scaffold Sybody of the convex library
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

    Plasmid

    #185678

    Purpose

    Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-RT001-LVB

    Plasmid

    #163381

    Purpose

    Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    LaG17-VEEGFRtm-BFP (LVB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge