-
Plasmid#176459PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1
Plasmid#197062PurposeDonor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)DepositorInsertsEncapsulin
Adaptor Protein
UseCRISPRTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1
Plasmid#197063PurposeDonor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)DepositorInsertsEncapsulin
Adaptor Protein
UseCRISPRTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_HH-HEK3 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA
Plasmid#176460PurposeVector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting HEK3 site driven by EF1alpha promoter and EGFP(L138S)-P2A-mCherry driven by CMVDepositorInsertHH-HEK3 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR
Plasmid#186744PurposeEF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backboneDepositorInsertEF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP
UseLentiviralTagsP2A-BFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
Plasmid#187612PurposeSplit luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassetteDepositorInsertsWWE domain (R163A) with C-terminal nLuc
WWE domain with C-terminal cLuc
UseLentiviralTagsFLAG, HA, Split luciferase (cLuc), Split lucifera…ExpressionMammalianMutationArg163AlaPromoterEF1AAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1
Plasmid#205445Purposehuman AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCTDepositorInsertiCasp9-Crry-mCD59-Qa1 SCT
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1
Plasmid#205443Purposehuman AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55DepositorInserthCD46-HLA-G SCT-hCD47-hCD59
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
XZ377 (PB) EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export-IRESv0-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export
Plasmid#233385PurposePiggyBac donor plasmid with optimized VEGF-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as markerDepositorInsertVEGFR1(ecto+TM)-ADAR2dd
UseTagsFLAG, HAExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ393 (PB) EF1a-IL2Rbsp-FLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export-IRESv0-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export
Plasmid#233388PurposePiggyBac donor plasmid with optimized IL-2-sensing eLIDAR receptor components separated by EMCV IRES, Puromycin resistance and BFP as markerDepositorInsertIL2Rb(ecto+TM)-ADAR2dd
UseTagsFLAGExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterEF1aAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-Luc-ZsGreen
Plasmid#39196DepositorInsertsFirefly Luciferase Luc2P
ZsGreen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1-alpha and EF1-alphaAvailable sinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
control (pGS701)
Plasmid#160037PurposepLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AV0.1-BWS-copGFP
Plasmid#124967PurposeExpress copGFP under EF1 promoterDepositorInsertcopGFP
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Nluc
Plasmid#73038PurposeExpress Nluc reporterDepositorInsertNluc
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
ER-J-TAG (pGS730)
Plasmid#160038PurposepLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsert3xHA-sfGFP-JPH-TMD
UseLentiviralTagsExpressionMutationPromoterEF1Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73037PurposeExpress Nluc and copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73040PurposeExpress Nluc and copGFP dual reporter in mammalian cellsDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterPGK (phosphoglycerate kinase 1)Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73039PurposeExpress Nluc, copGFP dual reporterDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancer and chicken bet…Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9
Plasmid#68416PurposeTransient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
C413-E40: attB4-EFSp>-attB1r
Plasmid#162928PurposeGateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoterDepositorInsertshort form of human EF1 alpha promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF_dCas9-VP64
Plasmid#68417PurposeTransient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.DepositorInsertdCas9
UseCRISPRTags3xFLAG and VP64ExpressionMammalianMutationD10A/H841APromoterhuman EF1[alpha]Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJP72
Plasmid#176479PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoterDepositorInsertsactin > GenR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP102
Plasmid#176480PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoterDepositorInsertsactin > NatR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP103
Plasmid#176481PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertsactin > HygR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH2.32_LV-mCherry-MS2x8-BCS
Plasmid#205539PurposeExpress mCherry-MS2x8 with barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-F-PA1
Plasmid#40618DepositorInsertPA1 (PAGR1 Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-UTY-F
Plasmid#40617DepositorInsertUTY (UTY Human)
UseLentiviralTagsFlagExpressionMutationPromoterEF1Available sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH2.156_LV-Zeo
Plasmid#205554PurposeExpress zeocin resistance markerDepositorInsertZeo
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAT005
Plasmid#171632Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-B2M
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRH623
Plasmid#171633Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a non-targeting sgRNA and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-non-targeting
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT003
Plasmid#171628Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-TRAC
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCAG and EF1-aAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR_mCherry
Plasmid#229078PurposeSmall lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterEF1sAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AV0-BWS-2.0
Plasmid#124976PurposeExpress gene of interest under EF1 promoterDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
LZ10 PBREBAC-H2BHalo
Plasmid#91564Purposepiggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.DepositorInserthistone H2B-HaloTag
UseTagsHaloTagExpressionMammalianMutationPromoterEF1Available sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1-a and U6Available sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH1-hPRMT7-GFP
Plasmid#87103Purposeexpress hPRMT7 in mammalian cellsDepositorInsertProtein arginine methyltransferase 7 (PRMT7 Human)
UseTagsExpressionMammalianMutationPromoterEF1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-mPRMT7-GFP
Plasmid#87104PurposeExpress mouse PRMT7 with Flag-tag in mammalian cellsDepositorInsertProtein arginine methyltransferase 7 (Prmt7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterEF1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
HSC1-D4Z4-GiP
Plasmid#58542PurposeDerived from HSC1-GiP where the D4Z4 insulator is cloned upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-SnxA
Plasmid#232460PurposeLentiviral EGFP lipid sensor for PI(3,5)P2DepositorInsertEGFP-SnxA
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWPI-myogenin
Plasmid#79073PurposeLentiviral plasmid that constitutively expresses human myogeninDepositorInserthuman myogenin (MYOG Human)
UseLentiviralTagsExpressionMutationPromoterEF1 alphaAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPExpressionMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-2xFYVE
Plasmid#136996PurposeLentiviral EGFP lipid sensor for PI(3)PDepositorInsertEGFP-2xFYVE
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
AV0-BWS-2.0-mC-G-CL3B
Plasmid#124975Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJG01
Plasmid#213505Purposeexpresses TdTomato in Capsaspora owczarzakiDepositorInsertTdTomato
UseTagsExpressionMutationPromoterEF1Available sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EGFP-P4M-SidMx2
Plasmid#136997PurposeLentiviral EGFP lipid sensor for PI(4)PDepositorInsertEGFP-P4M-SidMx2
UseLentiviralTagsEGFPExpressionMutationPromoterEF1 alphaAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_NS5_Flag
Plasmid#79639PurposeThird generation Self-inactivating lentivector expressing NS5 from Zika virusDepositorInsertNS5
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1Available sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_NS3_Flag
Plasmid#79635PurposeThird generation Self-inactivating lentivector expressing NS3 from Zika virusDepositorInsertNS3
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_NS2B_Flag
Plasmid#79637PurposeThird generation Self-inactivating lentivector expressing NS2B from Zika virusDepositorInsertNS2B
UseLentiviralTagsFlagExpressionMammalianMutationPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only