We narrowed to 18,088 results for: multi

1. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   OsU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. piggyBAC-5xTAPE-1-BlastR

   Plasmid

   #175808

   Purpose

   Encodes 5xTAPE-1 construct that can be integrated using piggyBAC system

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Insert

   5xTAPE-1 construct
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJIT163-eAFID-3

   Plasmid

   #167360

   Purpose

   For inducing predictable deletions in protoplasts and wheat plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3Bctd-Cas9-UDG-T2A-AP lyase
   

   Expression

   Plant

   Mutation

   WT

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIRECT_10A

   Plasmid

   #91207

   Purpose

   Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH-AFID-3

   Plasmid

   #167361

   Purpose

   For inducing predictable deletions in rice plants

   Depositor

   Caixia Gao

   Article

   Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

   Insert

   A3A-Cas9-UDG-T2A-AP lyase
   

   Expression

   Plant

   Mutation

   WT

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPV-EF1a-EGxxFP-iP-A

   Plasmid

   #204624

   Purpose

   Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-FAS-GCaMP6f-WPRE

   Plasmid

   #216277

   Purpose

   AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in neurons that express Cre recombinase)

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   GCaMP6f
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Promoter

   human synapsin 1 (hSyn)

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTC391

   Plasmid

   #91220

   Purpose

   protoplast vector expressing gRNA23 targeting tomato ANT1 (with TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIRECT_10E

   Plasmid

   #91209

   Purpose

   Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5 FRT/TO Plk4 ∆24-mCherry

    Plasmid

    #80271

    Purpose

    mammalian expression plasmid for Plk4 with multi-phospho degron deleted

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    Plk4 (PLK4 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    deletion of multi-phospho degron (amino acids 282…

    Promoter

    CMV

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJIT163-AFID-3

    Plasmid

    #167359

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG-T2A-AP lyase
    

    Expression

    Plant

    Mutation

    WT

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    AtU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pICH47802-p35S::tdTomato-ER::tNOS

    Plasmid

    #160220

    Purpose

    tdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverse

    Depositor

    Matias Kirst

    Article

    Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

    Insert

    p2x35S::TdTomato-ER::tNOS
    

    Use

    Synthetic Biology; Moclo toolkit level 1 acceptor…

    Expression

    Plant

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+ Gal4-GBP2-IRES-GBP7-p65

    Plasmid

    #50020

    Purpose

    Multi-purpose expression of a GFP-dependent transcription factor

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD
    

    Use

    Multi-purpose expression

    Promoter

    CMV-immediate early promoter and SP6 promoter

    Available Since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD_B2518

    Plasmid

    #91075

    Purpose

    Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    TaU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMOD_C3006

    Plasmid

    #91096

    Purpose

    Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Promoter

    FMV 34S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTC364

    Plasmid

    #91213

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYZ_3WJrep_N20_switch

    Plasmid

    #132722

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ_3WJrep_N20_trigger

    Plasmid

    #132726

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_trigger
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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