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Plasmid#212136PurposeCargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-sfgfp
Plasmid#209994PurposeContains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmidsDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL0_DE-Venus
Plasmid#202157PurposeLevel 0 plasmid containing a Venus fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertVenus gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_DE-sfGFP
Plasmid#202156PurposeLevel 0 plasmid containing a sfGFP fluorescent protein with DE overhangs used to build a level 1 construct.DepositorInsertsfGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eGFP
Plasmid#202149PurposeLevel 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-Venus
Plasmid#202148PurposeLevel 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertVenus gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pL0_CD-sfGFP
Plasmid#202147PurposeLevel 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertsfGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eCFP
Plasmid#202142PurposeLevel 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteCFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-roGFP
Plasmid#202140PurposeLevel 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInsertroGFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL0_CD-eYFP
Plasmid#202139PurposeLevel 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.DepositorInserteYFP gene
UseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_75_Donor_Tag_RPL13A-mTurq2
Plasmid#194415PurposePlasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRproDepositorInsertmTurquoise2 CDS
UseSynthetic Biology; Used as donor dna in genome in…TagsExpressionYeastMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBT0170
Plasmid#172813PurposeLevel 1 module containing nCFP_P4 FDepositorInsertp35S-5UTMV-SP_SV40-CFP-3U+TERNOS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBC0080
Plasmid#172786PurposeLevel 0 module containing NT2_nVenusDepositorInsert3X FLAG CtmVENUS 210-238 RSIAT pICH41258 (SP NT2)
UseSynthetic BiologyTagsExpressionMutationnoPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK4a_018
Plasmid#123831PurposeEncodes a nuclear localized mTurquoise2 coexpression cassette as a Type 4a part to be used in the MTK systemDepositorInsertP2a-NLS::mTurquoise
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF736
Plasmid#121893PurposeEF1a-dTEV-protease-GFPDepositorInsertsTEV protease
eGFP
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTCW022
Plasmid#83532Purposepheromone mediated expression of UBiC, ARO4, and TKL1DepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW015
Plasmid#83530PurposeUBiC and feedback resistant ARO4 expression from pheromone inducible FUS1J2 promoterDepositorInsertpFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t
UseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only