167,150 results

Showing: 841 - 860 of 167150 results

Results per page:

Sort results by:

mouse b6 full length

Plasmid

#217816

Purpose

Full length mouse integrin b6 expression

Depositor

Timothy Springer

Article

Yuxin et al bioRxiv 2024.01.26.577394

Insert

ITGB6 (Itgb6 Mouse)

Tags

P2A-mCherry

Expression

Mammalian

Promoter

CAG

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-GST-TEV-mCherry-TBK1

Plasmid

#198033

Purpose

Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV and mCherry

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2025

Insert

nucA

Tags

His and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized ecoli residues - 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRIS-PITChv2-FBL

Plasmid

#63672

Purpose

PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Depositor

Takashi Yamamoto

Article

Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

Insert

EGFP-2A-PuroR

Use

CRISPR

Promoter

Promoterless

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-NLS-HA-Bxb1

Plasmid

#51271

Purpose

Component of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

NLS-HA-Bxb1

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-nEF Con/Fon DREADD Gq-mCherry

Plasmid

#183532

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIG-601_NY-ESO-1_TCR_GFP

Plasmid

#207482

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

GFP, NY-ESO-1_TCR

Use

CRISPR

Mutation

Potential silent point mutation in GFP sequence

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Cre-IRES-PuroR

Plasmid

#30205

Purpose

Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

Depositor

Darrell Kotton

Article

Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

Insert

Cre recombinase (cre Enterobacteria phage P1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRiA4-VIR

Plasmid

#138193

Purpose

Virulence helper compatible with pCambia binary vectors.

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

vir gene cluster

Use

Vir gene expression

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p16sheLL

Plasmid

#37320

Depositor

John Schiller

Article

Buck et al PLoS Pathog. 2006 Jul;2(7):e69.

Insert

HPV16L1+L2

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-GGamma2-GFP2

Plasmid

#166774

Purpose

Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma2-GFP2 (GNG2 Human, Synthetic)

Expression

Mammalian

Mutation

Contains an N-terminal GFP2 and GSAG linker

Promoter

CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-RpHLuorin2

Plasmid

#171721

Purpose

GPI-anchored RpHLuorin2 for plasma membrane expression (extracellular)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to GPI anchor

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV enAsCas12f-HKRA-sgRNA_v7

Plasmid

#208357

Purpose

Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-HKRA

Expression

Mammalian

Mutation

I123H/D195K/D208R/V232A

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUltra-Hot-PC

Plasmid

#184466

Purpose

Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer20

Plasmid

#44012

Purpose

Tet-inducible lentiviral vector for ORF expression

Depositor

Stephen Elledge

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Type

Empty backbone

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-Ubiquitin

Plasmid

#18712

Purpose

Mammalian expression of human ubiquitin with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

Insert

ubiquitin (UBB Human)

Tags

HA

Expression

Mammalian

Mutation

The HA-Ubiquitin insert is from the UBB gene. The…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge