159,557 results

1. pAAV-EF1α-F-FLEX- Kir2.1-T2A-tdTomato

   Plasmid

   #60661

   Purpose

   Flp-dependent expression of Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   FLEX- Kir2.1-T2A-tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1α

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-Beclin1 (FL)

   Plasmid

   #24388

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Beclin1 (BECN1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF142_U6-sgRNA

   Plasmid

   #225960

   Purpose

   U6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.

   Depositor

   Christof Fellmann

   Article

   Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

   Insert

   U6-sgRNA (recipient)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-INSR-V5/HIS

   Plasmid

   #201980

   Purpose

   expression of human INSR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   INSR receptor tyrosine kinase (INSR Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CIP4-pmCherryC1

   Plasmid

   #27685

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   CIP4 (Trip10 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

6. Lenti-eCas9

   Plasmid

   #140237

   Purpose

   Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

   Depositor

   Urs Greber

   Article

   Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a core

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPD189 VP16-ZF37

   Plasmid

   #138759

   Purpose

   Constitutive expression of VP16-ZF37 under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VP16-ZF37
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDD112

   Plasmid

   #119893

   Purpose

   pNG2 backbone with an optimized chloramphenicol resitance marker for Rhodococcus opacus PD630

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRTpsiCHECK_Gaussia_luciferase

   Plasmid

   #154459

   Purpose

   Expression of gaussia luciferase and firefly luciferase

   Depositor

   Markus Landthaler

   Article

   Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

   Insert

   Gaussia luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPD543 ZF21x6-Compact_EYFP

    Plasmid

    #138857

    Purpose

    EYFP reporter vector for the ZF21x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF21x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF21x6-Compact

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPD528 VP16-ZF21

    Plasmid

    #138757

    Purpose

    Constitutive expression of VP16-ZF21 under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VP16-ZF21
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFPC1-EPSIN 1

    Plasmid

    #22228

    Depositor

    Pietro De Camilli

    Article

    Chen et al Nature. 1998 Aug 20. 394(6695):793-7.

    Insert

    Epsin 1 (Epn1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    please see depositor comment below

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-endo-EKAR4

    Plasmid

    #205513

    Purpose

    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-EKAR4
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Recall-miniCMV-sfGFP

    Plasmid

    #137995

    Purpose

    Backbone for generation of ClonMapper Recall Plasmid

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-HA_WT POLK

    Plasmid

    #201584

    Purpose

    Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    Polymerase kappa (POLK Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138131

    Purpose

    Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mCherry-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-PM-RA-BlastR

    Plasmid

    #211708

    Purpose

    Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    PM-targetted RA (Lck Synthetic)
    

    Use

    Lentiviral

    Tags

    RA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

    Plasmid

    #158741

    Purpose

    Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Hemagglutinin A/MI/45/15 H1N1
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863)

    Plasmid

    #199773

    Purpose

    enhancer AAV for expression of SYFP2 in midbrain dopamine neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    wtGFP plus mutations F46L F64L S65G S72A M153T V1…

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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