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4,272 results

Showing: 841 - 860 of 4272 results

  1. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  6. pCAG-VSVN

    Plasmid
    #64087
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain nucleoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV nucleoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64087-plasmid-map-sequence-id-436491

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  7. pSBtet-RP

    Plasmid
    #60497
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and puromycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60497-plasmid-map-sequence-id-97550

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  8. pCAG-VSVP

    Plasmid
    #64088
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV phosphoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64088-plasmid-map-sequence-id-438903

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  9. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  15. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Article
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Expression
    Bacterial
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    89439-plasmid-map-sequence-id-190157

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  16. pLV-EF1-Cre-PGK-Puro

    Plasmid
    #108543
    Purpose
    Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter
    Depositor
    Javier Alcudia
    Inserts
    cre recombinase
    Puromycin
    Promoter
    EF1apha and PGK
    Available Since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108543-plasmid-map-sequence-id-208672

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  19. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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Showing: 841 - 860 of 4272 results