We narrowed to 5,876 results for: nls

1. pY125 (pcDNA3.1-huBfCpf1)

   Plasmid

   #92301

   Purpose

   Expression of humanized BfCpf1

   Depositor

   Feng Zhang

   Insert

   huBfCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pY115 (pcDNA3.1-huMlCpf1)

   Plasmid

   #92275

   Purpose

   Expression of humanized MlCpf1

   Depositor

   Feng Zhang

   Insert

   huMlCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY112 (pcDNA3.1-huSaCpf1)

   Plasmid

   #92269

   Purpose

   Expression of humanized SaCpf1

   Depositor

   Feng Zhang

   Insert

   huSaCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-hA3A-eBE-Y130F

   Plasmid

   #113423

   Purpose

   Expresses hA3A-eBE-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpyCas9 PE2*-3HA

   Plasmid

   #169854

   Purpose

   Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-star-M-MLV-3HA
   

   Use

   Tags

   3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-hA3A-eBE-Y132D

   Plasmid

   #113429

   Purpose

   Expresses hA3A-eBE-Y132D in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y132D

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRD439

   Plasmid

   #168465

   Purpose

   For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mTurquoise2-H-NSdbd-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRD437

   Plasmid

   #168463

   Purpose

   For the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mEos3.2-H-NSdbd-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRD438

   Plasmid

   #168464

   Purpose

   For the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mEos3.2-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRD440

    Plasmid

    #168466

    Purpose

    For the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mCherry-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRD441

    Plasmid

    #168467

    Purpose

    For the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mEGFP-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRD442

    Plasmid

    #168468

    Purpose

    For the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-eYFP-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRD444

    Plasmid

    #168470

    Purpose

    For the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mNeonGreen-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid

    #92292

    Purpose

    Expression of humanized Mb2Cpf1

    Depositor

    Feng Zhang

    Insert

    huMb2Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid

    #92267

    Purpose

    Expression of humanized TsCpf1

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pY117 (pcDNA3.1-huMb3Cpf1)

    Plasmid

    #92293

    Purpose

    Expression of humanized Mb3Cpf1

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY113 (pcDNA3.1-huPb2Cpf1)

    Plasmid

    #92272

    Purpose

    Expression of humanized Pb2Cpf1

    Depositor

    Feng Zhang

    Insert

    huPb2Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pY119 (pcDNA3.1-huLb5Cpf1)

    Plasmid

    #92295

    Purpose

    Expression of humanized Lb5Cpf1

    Depositor

    Feng Zhang

    Insert

    huLb5Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pY118 (pcDNA3.1-huLb4Cpf1)

    Plasmid

    #92294

    Purpose

    Expression of humanized Lb4Cpf1

    Depositor

    Feng Zhang

    Insert

    huLb4Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pY124 (pcDNA3.1-huBsCpf1)

    Plasmid

    #92300

    Purpose

    Expression of humanized BsCpf1

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEBFP2-Nuc

    Plasmid

    #14893

    Purpose

    Mammalian expression of EBFP2 blue fluorescent protein with NLS

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 Apr 20. ():.

    Insert

    pEBFP2-Nuc
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pmKalama1-Nuc

    Plasmid

    #14894

    Purpose

    Mammalian expression of mKalama1 blue fluorescent protein with NLS

    Depositor

    Robert Campbell

    Article

    Ai et al Biochemistry. 2007 Apr 20. ():.

    Insert

    pmKalama1-Nuc
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    I178T mismatch, replace EYFP of the vector pEYFP-…

    Promoter

    Available since

    May 15, 2007

    Availability

    Academic Institutions and Nonprofits only

24. pCMV-104

    Plasmid

    #79842

    Purpose

    Expresses NLS-PpsR2-VP16 under CMV promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    NLS-PpsR2-VP16
    

    Use

    Tags

    Nuclear localization signal of nuclear cap-bindin…

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMV

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNB330

    Plasmid

    #60147

    Purpose

    Disintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    NLuc
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pNB774

    Plasmid

    #60114

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Unspecified

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNB690

    Plasmid

    #60134

    Purpose

    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pNB775

    Plasmid

    #60115

    Purpose

    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Unspecified

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNB776

    Plasmid

    #60117

    Purpose

    Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Use

    Unspecified

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNB754

    Plasmid

    #60157

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by LYS9 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    LYS9pr

    Available since

    Dec. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNB691

    Plasmid

    #60135

    Purpose

    Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    RdLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNB753

    Plasmid

    #60148

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCL33-decoder

    Plasmid

    #159451

    Purpose

    Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    Pfus1mut-tetR-nls-malE-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfus1mut

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNB689

    Plasmid

    #60133

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNB756

    Plasmid

    #60149

    Purpose

    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    YeLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNB752

    Plasmid

    #60155

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    ADE17pr

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mCerulean-Nucleus-12

    Plasmid

    #54556

    Purpose

    Localization: Nucleus, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mCerulean-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid

    #92296

    Purpose

    Expression of humanized FbCpf1

    Depositor

    Feng Zhang

    Insert

    huFbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pY126 (pcDNA3.1-huBoCpf1)

    Plasmid

    #92302

    Purpose

    Expression of humanized BoCpf1

    Depositor

    Feng Zhang

    Insert

    huBoCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pY123 (pcDNA3.1-huCMaCpf1)

    Plasmid

    #92299

    Purpose

    Expression of humanized CMaCpf1

    Depositor

    Feng Zhang

    Insert

    huCMaCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid

    #92273

    Purpose

    Expression of humanized PgCpf1

    Depositor

    Feng Zhang

    Insert

    huPgCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid

    #92298

    Purpose

    Expression of humanized CPbCpf1

    Depositor

    Feng Zhang

    Insert

    huCPbCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRDA174_EF1a_EnAsCas12a_BFP

    Plasmid

    #196724

    Purpose

    Sortable and selectable EnAsCas12a.

    Depositor

    Bernhard Schmierer

    Insert

    enASCas12a-HA-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS - HA and SV40 NLS

    Expression

    Mutation

    E174R, S542R, K548R

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BII-Sh-BnTW

    Plasmid

    #133365

    Purpose

    Piggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLS

    Depositor

    Blair Madison

    Insert

    tdTomato-NLS
    

    Use

    Tags

    Flag-tagged tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Promoter

    Available since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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47. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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48. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid

    #92306

    Purpose

    Expression of humanized BsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. mEmerald-Nucleus-7

    Plasmid

    #54206

    Purpose

    Localization: Nucleus, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Use

    Tags

    mEmerald-Nucleus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

50. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid

    #92304

    Purpose

    Expression of humanized Mb2Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb2Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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