165,567 results

1. pAG1186 Lyn11-ECFP-FIREmate

   Plasmid

   #205168

   Purpose

   Expresses Lyn11-ECFP-FIREmate in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   Lyn11-ECFP-FIREmate
   

   Expression

   Mammalian

   Available Since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. shLuc pRRL

   Plasmid

   #19125

   Depositor

   Bob Weinberg

   Article

   Godar et al Cell. 2008 Jul 11. 134(1):62-73.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-CAG::Cytbow

   Plasmid

   #158995

   Purpose

   Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Cytbow
   

   Tags

   The default expressed EBFP2 is fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. V5-BS2-NES (Cytosol)

   Plasmid

   #220341

   Purpose

   Mammalian expression vector BS2 in cytoplasm

   Depositor

   Bryan Dickinson

   Article

   Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

   Insert

   BS2
   

   Tags

   NES (Nuclear exclusion sequence) and V5 tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLenti PGK GFP Blast (w510-5)

   Plasmid

   #19069

   Purpose

   3rd gen lentiviral eGFP expression vector, PGK promoter, Blast

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

   Plasmid

   #183410

   Purpose

   piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   KRAB-dCas9-ecDHFR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-GG-gRNA

   Plasmid

   #226860

   Purpose

   Human gRNA expression vector backbone with BsmBI Golden Gate site

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   Sp-gRNA
   

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3 2 kb prom. CD274

   Plasmid

   #107003

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. alpha7nACHR_Nacho_mCherry

   Plasmid

   #182939

   Purpose

   alpha7 nACHR expression in mammalian cell lines

   Depositor

   Robert Brenner

   Article

   Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

   Insert

   NACH7 (CHRNA7 Human)
   

   Use

   Synthesized

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLM1110_LEU2

    Plasmid

    #168460

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    mRFP1
    

    Use

    Synthetic Biology; Yeast assembly vector for big-…

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGD-P1/HC-Pro

    Plasmid

    #196328

    Purpose

    35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

    Depositor

    Zhenghe Li

    Article

    Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

    Insert

    tobacco etch virus P1/HC-Pro
    

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-LKB1

    Plasmid

    #8590

    Depositor

    Lewis Cantley

    Article

    Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

    Insert

    LKB1 (STK11 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQC-FsCFP-mCherryFP

    Plasmid

    #154836

    Purpose

    Frame-shift fluorescent reporter for genomic DNA editing

    Depositor

    Fangliang Zhang

    Article

    Kumar et al BMC Genomics. 2019 Jul 24;20(1):609. doi

    Insert

    targeting sequence of VEGF
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pArcCreER

    Plasmid

    #46389

    Depositor

    Liqun Luo

    Article

    Guenthner et al Neuron. 2013 Jun 5;78(5):773-84. doi: 10

    Inserts

    Arc (Arc Mouse)
    CreERT2
    

    Use

    Mouse Targeting

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCJ217

    Plasmid

    #162685

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    G489C, S530C; codon optimized for E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA Raptor

    Plasmid

    #8513

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Tags

    ha

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBroad-T7RNAP-chlorR

    Plasmid

    #199992

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Expression

    Bacterial

    Mutation

    copFnull

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLSB-KAN

    Plasmid

    #166700

    Purpose

    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

    Depositor

    Robin Allshire

    Article

    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

    Inserts

    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    

    Expression

    Yeast

    Promoter

    adh15 promoter and tRNA Ser promoter

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Expression

    Bacterial

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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