159,557 results

1. pLVX-CMV-Galpha-q ONE-GO

   Plasmid

   #189738

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-q ONE-GO
   GNAQ (Gnaq Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSBtet-GP

   Plasmid

   #60495

   Purpose

   SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TCE & RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1/hChR2(H134R)-mCherry

   Plasmid

   #20938

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB_iMYOD1_P2A_BAF60C2_T2A_mCh_Puro

   Plasmid

   #168807

   Purpose

   PiggyBac transposon construct with doxycycline inducible MYOD1, BAF60C + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   MYOD1 (MYOD1 Synthetic, Human)
   

   Use

   Synthetic Biology; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pARF1-CFP

   Plasmid

   #11381

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   ADP Ribosylation Factor 1 (ARF1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR221_SLC2A3

   Plasmid

   #132114

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC2A3 (SLC2A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. phage-cmv-cfp-24xms2

   Plasmid

   #40651

   Depositor

   Robert Singer

   Article

   Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

   Insert

   CFP-24xMBS
   

   Use

   Lentiviral

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4-FLAG-Jhdm2a

   Plasmid

   #38136

   Depositor

   Toru Nakano, Toshinobu Nakamura

   Article

   Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

   Insert

   Jhdm2a (Kdm3a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   protein starts at aa115

   Promoter

   CMV

   Available since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA5/FRT-ChemoG-CaM

   Plasmid

   #193816

   Purpose

   CMV overexpression of ChemoG-CaM in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG-CaM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:E116D, A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G9A[SET]-dCas9

    Plasmid

    #100089

    Purpose

    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    G9A (EHMT2 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-G9A[SET]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    G9A[SET] includes aa 829-1209

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-WT-STAT1

    Plasmid

    #71454

    Purpose

    Lentiviral expression of WT-STAT1

    Depositor

    George Stark

    Article

    Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-Cre-GFP

    Plasmid

    #68544

    Purpose

    AAV expression of Cre-GFP fusion

    Depositor

    Eric Nestler

    Article

    Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-RhoA

    Plasmid

    #23224

    Depositor

    Channing Der

    Article

    Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pTP284

    Plasmid

    #113312

    Purpose

    human Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG-JAK1

    Plasmid

    #174572

    Purpose

    Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    JAK1 (JAK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYS28

    Plasmid

    #202085

    Purpose

    Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    CT Halo-T2A-HygR Minicircle Parent
    

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    None

    Available since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    mDlx

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge