We narrowed to 75,869 results for: Rest

1. pCMycDam

   Plasmid

   #59218

   Purpose

   Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam

   Depositor

   Bas van Steensel

   Article

   van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

   Type

   Empty backbone

   Use

   Damid

   Tags

   Dam and Myc

   Expression

   Insect

   Mutation

   Promoter

   Drosophila heat-shock promoter

   Available since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS2-2

   Plasmid

   #220628

   Purpose

   Plasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1-N-Myc

   Plasmid

   #119757

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFKBPmCherry-Akt2(KD)

   Plasmid

   #86598

   Purpose

   Expresses recruitable mCherry-tagged kinase domain of human Akt2(WT)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   FKBP-mCherry-Akt2(KD)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHaloTag-PS(RA)

   Plasmid

   #86627

   Purpose

   Expresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTag

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   HaloTag-PS(RA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. H-ACNF-H

   Plasmid

   #15515

   Depositor

   Peter Mombaerts

   Article

   Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

   Insert

   H element
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Targeting vector to delete the 2,119 nt sequence,…

   Promoter

   Available since

   Aug. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pBlue-hFX-HA(AscI)

   Plasmid

   #15404

   Depositor

   Michael Ristow

   Insert

   human frataxin cDNA (FXN Human)
   

   Use

   Tags

   AU1 and HA

   Expression

   Bacterial

   Mutation

   700 bp insert can be released by digestion with r…

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL1530

   Plasmid

   #226636

   Purpose

   This plasmid is for the induced expression of nrsB (formerly known as ORF229) from ICP1_2001.

   Depositor

   Andrew Camilli

   Article

   Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

   Insert

   nrsB (ORF 229)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. POC1400

   Plasmid

   #213121

   Purpose

   Testing the activity of BpiI-associated switch methylases

   Depositor

   Christopher A. O'Callaghan

   Article

   Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. POC1401

    Plasmid

    #213122

    Purpose

    Testing the activity of LguI-associated switch methylases

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. POC1399

    Plasmid

    #213120

    Purpose

    Testing the activity of BsaI-associated switch methylases

    Depositor

    Christopher A. O'Callaghan

    Article

    Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3 RARE-d2EGFP

    Plasmid

    #183055

    Purpose

    Destabilized EGFP fluorescent reporter for Retinoic Acid activity

    Depositor

    Chaya Kalcheim

    Article

    Rekler et al Elife. 2022 Apr 8;11. pii: 72723. doi: 1

    Insert

    d2EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    RARE, SV40

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pABY-T2

    Plasmid

    #201765

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIS-GALkFLP(Trp1)

    Plasmid

    #49459

    Purpose

    overespression of Flp step-arrest mutant in yeast, TRP1 selection

    Depositor

    Marc Gartenberg

    Article

    Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    GAL10

    Available since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIII/MS2-2

    Plasmid

    #220630

    Purpose

    For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL20C-ddIPpoI

    Plasmid

    #49053

    Purpose

    Expresses the restriction enzyme IPpoI under the control of Shield1

    Depositor

    Michael Kastan

    Article

    Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

    Insert

    DD-HA-ER-IPpoI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pABY-T1

    Plasmid

    #201764

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUPD2-HR1b-GFP-L (B3-B4)

    Plasmid

    #211853

    Purpose

    E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. BsaI-tagRFP-BsaI-pUC-kan

    Plasmid

    #170771

    Purpose

    test plasmid for Golden Gate assembly, encodes fluorescent red protein

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    tagRFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDL1403

    Plasmid

    #226635

    Purpose

    This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

    Depositor

    Andrew Camilli

    Article

    Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

    Inserts

    tfox
    qstR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Halo-TMD

    Plasmid

    #195347

    Purpose

    Fluorescent labeling for cell membranes

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pABY-T3

    Plasmid

    #201766

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pABY-T4

    Plasmid

    #201767

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFGL815

    Plasmid

    #52322

    Purpose

    A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.

    Depositor

    Naweed Naqvi

    Article

    Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10

    Type

    Empty backbone

    Use

    Agrobacterium

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMtBS

    Plasmid

    #32226

    Depositor

    Christopher Buck

    Article

    Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

    Insert

    MCV Small t antigen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Splice site was silently mutated, and a restricti…

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAllet

    Plasmid

    #202560

    Purpose

    E. coli cloning vector with large MCS (50+ unique restriction sites)

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. DinoIII

    Plasmid

    #128100

    Purpose

    Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.

    Depositor

    Senjie Lin

    Article

    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

    Insert

    RNA complex & upstream/downstream of LHC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Inserts are potential promoters

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pID3.01B

    Plasmid

    #22720

    Depositor

    Geraldine Seydoux

    Article

    D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

    Insert

    unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA 3.1(-) TRIM12A

    Plasmid

    #87061

    Purpose

    Murine homologues of human Trim5

    Depositor

    Jeremy Luban

    Article

    Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

    Insert

    TRIM12A (Trim12a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA 3.1(-) TRIM12C

    Plasmid

    #87080

    Purpose

    Murine homologues of human Trim5

    Depositor

    Jeremy Luban

    Article

    Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

    Insert

    TRIM12C (Trim12c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCBLggCDS

    Plasmid

    #199737

    Purpose

    Golden Gate entry vector for gene of interest cloning

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDL1531

    Plasmid

    #226637

    Purpose

    This plasmid is for correcting the nonsense mutation in the nrsB gene of several ICP1 isolates.

    Depositor

    Andrew Camilli

    Article

    Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

    Insert

    Upstream sequence of nrsB (only 338bp total)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGAT

    Plasmid

    #112587

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA 3.1(-) TRIM30B

    Plasmid

    #87082

    Purpose

    Murine homologues of human Trim5

    Depositor

    Jeremy Luban

    Article

    Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

    Insert

    TRIM30B (Trim30b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmCherry-PH(Btk)KD(Akt1)

    Plasmid

    #86636

    Purpose

    Expresses mCherry-tagged chimeric fusion of Btk PH domain with the kinase domain of human Akt1

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    PH(Btk)KD(Akt1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRL093 pattB_exorh:EGFP_MCS

    Plasmid

    #213514

    Purpose

    attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHIV-EGFP

    Plasmid

    #21373

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

    Depositor

    Bryan Welm, Zena Werb

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. MscL(G22S)61-cpGFP-ERexp

    Plasmid

    #195311

    Purpose

    Fluorescent reporter/biosensor for membrane tension

    Depositor

    Allen Liu

    Article

    Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

    Insert

    MscL(G22S)61-cpGFP-ERexp
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge