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pCITF-KCC2

Plasmid

#61404

Purpose

Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

Depositor

Franck Polleux

Article

Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

Insert

KCC2 (SLC12A5 Human)

Tags

IRES-tdTomato-Flag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLGB29

Plasmid

#126619

Purpose

Suicide vector for allelic replacement in Bacteroides, inulin selection and aTC-inducible ss-Bfe1 counterselection

Depositor

Laurie Comstock

Article

Garcia-Bayona et al mBio. 2019 Aug 13;10(4). pii: mBio.01762

Type

Empty backbone

Use

Allellic exchange in bacteria

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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eMagBF-TgRFPT

Plasmid

#162253

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagBF-TgRFPT

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRL128

Plasmid

#40180

Depositor

Eric Cascales

Article

Gueguen et al Appl Environ Microbiol. 2013 Jan;79(1):3

Inserts

Ptac-lacO
araC-pBAD

Promoter

BAD and tac

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Autobow

Plasmid

#45182

Depositor

Joshua Sanes

Article

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Autobow

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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GST-14-3-3wt (pEBG)

Plasmid

#1942

Depositor

Joseph Avruch

Article

Tzivion et al Nature 1998 Jul 2;394(6688):88-92.

Insert

14-3-3 zeta (Ywhaz Mouse)

Tags

GST

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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FUGW-PK-hLC3∆G

Plasmid

#61461

Purpose

Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3∆G (MAP1LC3B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hUBC

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Article

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A Human, (*see below))
humanized birA

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mem-TurboID

Plasmid

#149409

Purpose

Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBbA8k-RFP

Plasmid

#35273

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

BAD

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 K52R

Plasmid

#17643

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

The lysine residue at position 52 was changed to …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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PKD1-F (pCI-PKD1-Flag)

Plasmid

#21369

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-RPS6KA3

Plasmid

#23527

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA3 (RPS6KA3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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