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Showing: 2201 - 2250 of 6304 results

  1. AAV-SYN-eGFP-oPRE

    Plasmid
    #218681
    Purpose
    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    eGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218681-plasmid-map-sequence-id-433352

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  2. pGS96_EGFP(A206K)-I3-01 AAVS donor

    Plasmid
    #211865
    Purpose
    I3-01 transgene integration at AAVS1 safe harbor locus
    Depositor
    Iain Cheeseman
    Insert
    I3-01
    Use
    CRISPR
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    211865-plasmid-map-sequence-id-426339

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  10. pAAV-U6-sgCTG-CMV-GFP

    Plasmid
    #216734
    Purpose
    AAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    sgCTG
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and CMV
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216734-plasmid-map-sequence-id-430319

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  11. pAAV-nEF-SpCas9D10A nickase

    Plasmid
    #216736
    Purpose
    Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)
    Depositor
    Vincent Dion
    Article
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    EF-1-alpha core
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216736-plasmid-map-sequence-id-430323

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  13. AAV-CAG-K-SPOTIT(R271F)

    Plasmid
    #191492
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT(R271F)
    Use
    AAV
    Tags
    Expression
    Mutation
    R271F in OR
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191492-plasmid-map-sequence-id-377952

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  14. AAV-CAG-M-SPOTIT2(R279F)

    Plasmid
    #191493
    Purpose
    Green fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    M-SPOTIT2(R279F)
    Use
    AAV
    Tags
    Expression
    Mutation
    R279F in OR
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191493-plasmid-map-sequence-id-377870

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  20. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

    Plasmid
    #211905
    Purpose
    Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.
    Depositor
    Yannick Doyon
    Article
    Levesque et al bioRxiv 2023.09.14.557485
    Insert
    DARIC-CD19-scFv
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    211905-plasmid-map-sequence-id-420042

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  28. AAV-sCAG-eGFP-RAB11

    Plasmid
    #203799
    Purpose
    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    203799-plasmid-map-sequence-id-413679

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  32. pAAV-hSyn-DIO-GRAB_CRF0.5

    Plasmid
    #208660
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208660-plasmid-map-sequence-id-412159

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  33. AAV-sCAG-eGFP-ACAP1

    Plasmid
    #203800
    Purpose
    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    203800-plasmid-map-sequence-id-410892

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  35. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Available since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  40. AAV1-DIO-eGFP-pvRPL10a

    Plasmid
    #202542
    Purpose
    Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.
    Depositor
    Zoe Donaldson
    Article
    Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75
    Insert
    RPL10a subunit of the ribosome
    Use
    AAV and Cre/Lox
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    optimized for prairie vole DNA sequence and optim…
    Promoter
    human synapsin
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    202542-plasmid-map-sequence-id-405350

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  48. pAAV2-UbC-3xNLS-mScarlet-I

    Plasmid
    #191099
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Pantelis Tsoulfas, Murray Blackmore
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet-I
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    optimized to human codon usage
    Promoter
    UbC
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191099-plasmid-map-sequence-id-380113

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Showing: 2201 - 2250 of 6304 results