162,408 results
-
Plasmid#222425PurposeTo express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cellsDepositorInsertLBR(1-245aa) (LBR Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncation (Includes aa 1-245)PromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1161
Plasmid#217964PurposeFor RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFP, APEXExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1177
Plasmid#217961PurposeDual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.DepositorInsertHistone H3 (HIST2H3PS2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAND1-T2A-dTom-PGK-Neo
Plasmid#209838PurposeTomato reporter plasmid targeting the human HAND1 geneDepositorInsertHAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
UseHuman targetingTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper_AraC/pBad-Tnpase (camR)
Plasmid#213909PurposeArabinose-inducible himar1C9 transposase expression - cam resistanceDepositorInsertAraC/pBad ATc-inducible himar1C9 transposase
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF1672
Plasmid#142823PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTP73 (TP73 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-HLA-B-COMP5AP-AviTag-9xHis
Plasmid#157399PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertHLA-B (HLA-B Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ADGRA3-Fc(DAPA)-AviTag-6xHis
Plasmid#156959PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertADGRA3 (ADGRA3 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-ADGRA2-Fc(DAPA)-AviTag-6xHis
Plasmid#156964PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertADGRA2 (ADGRA2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-halo-Oct4
Plasmid#197909Purposesingle-particle tracking for OCT4DepositorInsertpou5f1 (Pou5f1 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMYT013
Plasmid#180666PurposeEncodes pTet5 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertpTet5
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH628_pJH_LldR_PlldR-sfGFP.3TAG
Plasmid#163727PurposeLactate-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertlldr gfp.3TAG
UseTagsExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-151a-3p
Plasmid#103258PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-151a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-151a-3p target (MIR151A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-151a-5p
Plasmid#103259PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-151a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-151a-5p target (MIR151A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-3p
Plasmid#103147PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-3p target (MIRLET7B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Integrin-Beta1-N-18
Plasmid#54129PurposeLocalization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertIntegrin-Beta1 (ITGB1 Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-Pom121 GFP
Plasmid#187680PurposeIn vivo visualization of NPC protein Pom121DepositorInsertPom121 (Pom121 Mouse)
UseTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIPK1 gamma 90
Plasmid#22299DepositorInserthuman phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
Myc-PSF-WT
Plasmid#35183DepositorInsertPSF (SFPQ Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -