159,589 results

1. pD454-SR mouse Alpha-synuclein

   Plasmid

   #89075

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_SMAD5_WT

   Plasmid

   #82217

   Purpose

   Gateway Donor vector containing SMAD5 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMAD5 (SMAD5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX-EF1a_GFP

   Plasmid

   #221481

   Purpose

   GFP lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CuO-V5 CASC3 siRes1+2 f.l. WT

   Plasmid

   #158540

   Purpose

   For PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3

   Depositor

   Niels Gehring

   Article

   Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

   Insert

   CASC3; BTZ; MLN51 (CASC3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Synapto-pHluorin

   Plasmid

   #168510

   Purpose

   Expression of Synapto-pHluorin for optical monitoring of presynaptic release in neurons.

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   Synaptophysin-pHluorin (Syp Mouse)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK290 (mAID-mClover-Hygro)

   Plasmid

   #72828

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Hygro
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL7.0: tgRFPt-SSPB WT

   Plasmid

   #60415

   Purpose

   Mammalian Expression of tgRFPt-Nano

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   tgRFPt-SSPB
   

   Use

   Cre/Lox and Lentiviral

   Tags

   TagRFPt

   Expression

   Mammalian

   Mutation

   Y11K and A15E

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

   Plasmid

   #128424

   Purpose

   Human Codon Optimized dCas9-DNMT3A3L

   Depositor

   Bradley Bernstein

   Article

   Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

   Insert

   dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1884

    Plasmid

    #143984

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZIC2 (ZIC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-R-GECO1.2

    Plasmid

    #45494

    Purpose

    Red intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Neurosci. 2013 Mar 19.

    Insert

    R-GECO1.2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

    Promoter

    CMV

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4-FLAG-Jhdm2a

    Plasmid

    #38136

    Depositor

    Toru Nakano, Toshinobu Nakamura

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMAL-p5X

    Plasmid

    #150814

    Purpose

    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein

    Depositor

    Paul Riggs

    Type

    Empty backbone

    Use

    Tags

    malE A313V

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 22, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDONR221_SLC2A3

    Plasmid

    #132114

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC2A3 (SLC2A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pSLIK CA RhoA

    Plasmid

    #112894

    Purpose

    promoter-inducible expression of constitutively-active RhoA

    Depositor

    Sanjay Kumar

    Article

    MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

    Insert

    CA RhoA (RHOA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF6-myc-TBC1D15

    Plasmid

    #79148

    Purpose

    mammalian expression of TBC1D15

    Depositor

    Aimee Edinger

    Article

    Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

    Insert

    TBC1D15 (Tbc1d15 Mouse)
    

    Use

    Tags

    His, Myc, and V5

    Expression

    Mammalian

    Mutation

    S616N (please see depositor comment below)

    Promoter

    EF-1alpha

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP284

    Plasmid

    #113312

    Purpose

    human Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Myler et al Mol Cell. 2017 Sep 7;67(5):891-898.e4. d

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SETD2-GFP

    Plasmid

    #80653

    Purpose

    mammalian expression of SETD2-GFP

    Depositor

    Sérgio De Almeida

    Article

    Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

    Insert

    SETD2 (SETD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3_mScarlet-LAMP1

    Plasmid

    #185138

    Purpose

    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

    Depositor

    Andrew York

    Article

    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

    Insert

    Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-21 of LAMP1

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PRDX6A-c001

    Plasmid

    #175474

    Purpose

    Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

    Depositor

    Opher Gileadi

    Insert

    PRDX6 (PRDX6 Human)
    

    Use

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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