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pSMALB-ATF4.5

Plasmid

#155032

Purpose

Fluorescent reporter for ATF4 translation

Depositor

John Dick, Peter van Galen

Article

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AAV Syn-coca-5HT3-IRES-mCherry

Plasmid

#242197

Purpose

AAV vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-coca-5HT3-IRES-mCherry (Htr3a Mouse)

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM63A-W472K-peGFP-N1

Plasmid

#228591

Purpose

Expresses mouse TMEM63A-W472K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM63A/CSC1-like protein 1 isoform 1 (Tmem63a Mouse)

Expression

Mammalian

Mutation

W472K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

Plasmid

#131683

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Txnip(C247S)

Plasmid

#206353

Purpose

AAV plasmid expressing mutant TXNIP

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

Txnip (Txnip Mouse)

Use

AAV

Mutation

C247S

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-cKit

Plasmid

#114293

Purpose

TET-inducible expression of c-Kit

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

cKit (Kit Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin-S3D

Plasmid

#78297

Purpose

Mammalian expression of mouse cofilin S3D fused to EGFP

Depositor

Michael Frotscher

Article

Chai et al Development. 2016 Mar 15;143(6):1029-40.

Insert

cofilin 1 (Cfl1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

S3D

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-S255D/T259E (GST)

Plasmid

#21566

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Aspartic Acid, Threonine 259 to Gl…

Available Since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pET23a-mUbc9

Plasmid

#53137

Purpose

E.coli expression of full length untagged mouse Ubc9 (protein sequence identical to human)

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

Ubc9 (Ube2i Mouse)

Tags

untagged

Expression

Bacterial

Mutation

protein sequence is identical to human

Promoter

T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-emiRFP670-HO1

Plasmid

#197252

Purpose

Expresses the protein of emiRFP670 and HO1 in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP670

Expression

Bacterial

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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myc-KIF5B

Plasmid

#127617

Purpose

N-terminal myc-tagged mouse KIF5B

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5B (Kif5b Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_

Plasmid

#201174

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6/sgClta-sfGFP-Clta

Plasmid

#200261

Purpose

U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

Depositor

Justin Eyquem

Article

Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

Insert

sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta

Use

AAV

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Brachyury-eGFP

Plasmid

#21063

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

Brachyury promoter

Use

Lentiviral

Tags

eGFP

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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WT NFAT1

Plasmid

#11100

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT1 (Nfatc2 Mouse)

Use

Retroviral

Tags

3xHA and GFP

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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eGFP-TRF1 pWzl-Hygro

Plasmid

#19834

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

Telomere-Repeat binding Factor 1 (Terf1 Mouse)

Use

Retroviral

Tags

enhanced GFP

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pL6puro-JeT-pmRevErbA-Venus-NLS-Pest

Plasmid

#240106

Purpose

A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added JeT Promoter

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest

Plasmid

#240112

Purpose

A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.

Depositor

Achim Kramer

Article

Gabriel et al bioRxiv 2025.06.30.661926

Insert

murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)

Use

Lentiviral

Mutation

added SV40-Enhancer

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox9

Plasmid

#36979

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only

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