We narrowed to 6,078 results for: nls

1. pcDNA3-BG4-NLS-mCer

   Plasmid

   #211448

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Use

   Tags

   NLS-mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS613_pTetR-P2A-BFPnls/sgTelo

   Plasmid

   #108649

   Purpose

   Telomere-targeting Spy sgRNA under U6 promoter

   Depositor

   Erik Sontheimer

   Article

   Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

   Inserts

   Telomere-targeting sgRNA
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-S5E6-dTom-nlsdTom

   Plasmid

   #135641

   Purpose

   AAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV1

   Insert

   dTom-nlsdTom
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-mCherry-CRY2-NLS

   Plasmid

   #145259

   Purpose

   Mammalian Expression of Nuclear Control OptoConstruct with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHsp83-NLS-HA-2xPCP-2xGFP

   Plasmid

   #71243

   Purpose

   Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   2xPCP-2xGFP
   

   Use

   P element-mediated germline transformation

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Hsp83

   Available since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-MBP-NLS-Geo_st

   Plasmid

   #87703

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Use

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CSII-prEF1a-mCherry-3xNLS

   Plasmid

   #125262

   Purpose

   Fluorescent reporter for cell size

   Depositor

   Jan Skotheim

   Article

   Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

   Insert

   mCherry-3xNLS
   

   Use

   Lentiviral

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-FNLSNG-P2A-Puro

   Plasmid

   #136900

   Purpose

   Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSNG
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Expression

   Mutation

   L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

   Promoter

   EF1s

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-Peredox-mCherry-NLS

    Plasmid

    #32385

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Use

    Retroviral

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Use

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 764 pME nls-mApple

    Plasmid

    #195962

    Purpose

    Gateway pME fluorophore

    Depositor

    Kristen Kwan, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mApple nuclear
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUBC-SV40NLS-Pfv-Sapphire

    Plasmid

    #203653

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-NS3-NLS/VPR

    Plasmid

    #112244

    Purpose

    Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

    Depositor

    John Ngo

    Article

    Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

    Insert

    dCas9-NS3-NLS/VPR
    

    Use

    Tags

    AU1 (N term on NS3) and HA (C term on NS3)

    Expression

    Mammalian

    Mutation

    NS3 mutant T54A

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn1-2xNLS-mTurquoise2

    Plasmid

    #118025

    Purpose

    An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    2xNLS-mTurquoise2
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    hSyn1

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-TetOn-NLS-mCherry

    Plasmid

    #193762

    Purpose

    Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    NLS-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    SV40 NLS fused to mCherry

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Akt-STOPS-NLS

    Plasmid

    #175007

    Purpose

    Nuclear-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-STOPS-NLS
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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