We narrowed to 6,501 results for: nls
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Plasmid#119773PurposeConversion of C to T in potatoDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191797Purposevector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized dCas13d
UseCRISPRExpressionMammalianMutationR239A, H244A, R858A, H863APromoterCBh, SV40, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag
Plasmid#132400Purposeoverexpression in human cellsDepositorInsertdPguCas13b
UseLentiviralTagsflagExpressionMammalianMutationH151A; H1121AAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
xCas9(3.6)-BE4
Plasmid#108386PurposeMammalian expression vector for xCas9(3.6)-BE4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertxCas9(3.6)-BE4
TagsNLSExpressionMammalianMutationE108G, S217A, A262T, S409I, E480K, E543D, M694I, …Available sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMVd1-NLS-GAL4-iLight-VP16
Plasmid#219549PurposeNLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.DepositorInsertNLS-Gal4(1-65)-iLight-VP16
UseAAVExpressionMammalianPromoterCMVd1Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK mCh-V5-NES-EGFP-SV40 NLS zeo
Plasmid#192340PurposeLentiviral expression vector for an inducible mCh-V5-NES-EGFP-SV40 NLSDepositorInsertmCh-V5-NES-EGFP-SV40 NLS
UseLentiviralTags3xFLAGAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP315
Plasmid#134994PurposeKRAB-m6A Reader. Repress transcription near Gm6ATC sites. pUBC-KRAB-NLS-DpnI(aa146-254)DepositorInsertpUBC-KRAB-NLS-DpnI(aa146-254)
TagsDpnI(aa146-254) and KRAB domainExpressionMammalianAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ14731-PHR-SFFV-NES*3-PYL1-pHintein-NLS-Cas9-NLS
Plasmid#252644PurposeHuman expression vector containing SFFV promoter, Cas9 fused to PYL1 and pH inteinDepositorInsertNES*3-PYL1-pHintein-NLS-Cas9-NLS
UseLentiviralPromoterSFFVAvailable sinceJune 22, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#69496PurposepJL1 expression vector with sfGFPDepositorInsertSuper folder GFP
ExpressionBacterialPromoterT7Available sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMEH275 NLS dTomato
Plasmid#168210PurposeMammalian expression of nuclear-localized dTomatoDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-mTurquoise2-NLS
Plasmid#165443PurposeExpresses nuclear mTurquoise2 from CMV promoterDepositorInsertmTurquoise2-NLS
UseAAVTagsNLSExpressionMammalianPromoterCMVAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR207-Citrine-NLS
Plasmid#44586PurposeGateway entry vector for Citrine fluorescent protein with a nuclear localization signalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCitrine-NLS
TagsNLS (Nuclear Localization Signal)Available sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB102-Citrine-NLS
Plasmid#79136PurposeBinary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plantsDepositorInsertCitrine-NLS
TagsNLS (Nuclear Localization Signal)ExpressionPlantPromoterCaMV 35S promoterAvailable sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47454PurposeepiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.DepositorInsertCRY2PHR-NLS-SID4X
UseAAVTags2A_phiLOV2.1ExpressionMammalianPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSB-TRE-CRISPRoff-EF1A-TetOn
Plasmid#203355PurposeDrug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backboneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPRoff-TetOn
Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH
Plasmid#178960PurposeExpresses saCas9 protein specifically in astrocytesDepositorInsertStaphylococcus aureus Cas9 (SaCas9)
UseAAVTags3x HAPromotergfaABC1D (astrocyte specific)Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME nls EGFP WPRE bGH pA (JDW 488)
Plasmid#224540PurposeA Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleusDepositorInsertnls-EGFP-WPRE-bGH-pA
UseGateway CloningAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-mTagBFP2-pA (JDW 1361)
Plasmid#224535PurposeA Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pADepositorInsertIRES-FLAG-NLS-mTagBFP2-pA
UseGateway CloningAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L
Plasmid#121840PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sitesDepositorTypeEmpty backboneUseGateway Cloning and Synthetic BiologyAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CSII-prEF1a-E2-Crimson-NLS
Plasmid#125263PurposeFluorescent reporter for cell sizeDepositorInsertE2-Crimson-NLS
UseLentiviralTagsNLSExpressionMammalianPromoterEF1aAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by