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GFP-JNK2

Plasmid

#86831

Purpose

mammalian expression of GFP-JNK2

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

JNK2 (MAPK9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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CAG::Gdnf

Plasmid

#78209

Purpose

piggybac vector for mouse Gdnf expression

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Gdnf (Gdnf Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-AID-PcrA-UGI

Plasmid

#221220

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-PcrA-UGI

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pIN1 (gpdA-cTerm-mGL-ptrA)

Plasmid

#188735

Purpose

Expresses mGreenlantern for C-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mGreenlantern PtrA

Use

Filamentous fungal expression

Tags

mGreenlantern

Promoter

gpdA

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pIN2 (gpdA-nTerm-mGL-ptrA)

Plasmid

#188736

Purpose

Expresses mGreenlantern for N-terminal tagging

Depositor

Michael Bromley

Article

Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

Insert

gdpA-mGreenlantern PtrA

Use

Filamentous fungal expression

Tags

mGreenlantern

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD IFP1.4

Plasmid

#91775

Purpose

Bacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4)

Depositor

Erik Rodriguez, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Insert

Infrared Fluorescent Protein 1.4 + Heme oxygenase-1

Tags

Hexa-Histidine tag

Expression

Bacterial

Promoter

araBAD

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO human ULK2 shRNA 91

Plasmid

#27634

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

human ULK2 shRNA 91

Use

Lentiviral

Expression

Mammalian

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only
pAG496

Plasmid

#226750

Purpose

Expresses exogenous MTCH2

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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mouse CENP-B-EGFP

Plasmid

#107264

Purpose

mouse CENP-B-EGFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

CENP-B-EGFP (Cenpb Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

T7

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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SSTR4-DuET

Plasmid

#213379

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

SSTR4 (SSTR4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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B-MFF

Plasmid

#141158

Purpose

Dimerization-dependent fluorescent protein binding partner targeted to outer mitochondrial membrane

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

mitochondrial fission factor (MFF Human)

Tags

GB

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GFP Snail 6SA

Plasmid

#16228

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

6SA: S97A, S101A, S108A, S112A, S116A, S120A

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-p38alpha

Plasmid

#47575

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

p38alpha (Mapk14 Mouse)

Tags

3xFlag and GST

Expression

Bacterial

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2_mSTING_scrambled_gRNA_2

Plasmid

#196628

Purpose

Scrambled control 2 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 2

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE-Tight-BI-Gl NORM-LacZA-TER-LacZB

Plasmid

#86194

Purpose

beta Globin NMD construct with and without PTC under Bi-directional TET on promoter

Depositor

Robert Singer

Article

Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

Insert

beta Globin (HBB, Hbb Human, Mouse)

Use

Tet on

Expression

Mammalian

Mutation

39TER in beta Globin in MCS II

Promoter

Tet on

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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plenti2-EF1a-ChR2-EYFP-WRPE

Plasmid

#125255

Purpose

3rd gen lentiviral expression of ChR2 with mutation fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-MBD1

Plasmid

#47057

Purpose

Expresses methyl-CpG binding domain protein 1

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Methyl-CpG Binding Domain Protein 1 (MBD1 Human)

Use

Gateway entry vector

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pLC-EGFP-RIP3

Plasmid

#75165

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIP3

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIP3 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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