We narrowed to 28,500 results for: REP

1. pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Plasmid

   #101217

   Purpose

   Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…

   Promoter

   T7

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW-TetO-Lin28

   Plasmid

   #60345

   Purpose

   Mammalian Expression, Lentiviral

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Lin28 (Lin28a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV + TetO

   Available since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Plasmid

   #101211

   Purpose

   Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Plasmid

   #101204

   Purpose

   Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-miR-195-3p

   Plasmid

   #103311

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-195-3p target (MIR195 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-hs-CDC34

   Plasmid

   #52731

   Purpose

   retroviral expression of human CDC34

   Depositor

   Shinya Yamanaka

   Article

   Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

   Insert

   CDC34 (CDC34 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. FL-BCL2L1

   Plasmid

   #78860

   Purpose

   DULIP positive control (prey) for assay establishment

   Depositor

   Erich Wanker

   Article

   Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

   Insert

   BCL2 like 1 (BCL2L1 Human)
   

   Use

   Expression vector

   Tags

   Firefly luciferase, V5-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. LSB-hsa-miR-30a-3p

   Plasmid

   #103409

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-30a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-30a-3p target (MIR30A Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LSB-hsa-miR-296-3p

   Plasmid

   #103387

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-296-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-296-3p target (MIR296 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LSB-hsa-let-7a-3p

    Plasmid

    #103145

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7a-3p target (MIRLET7A3 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LSB-hsa-miR-146a-3p

    Plasmid

    #103247

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-146a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-146a-3p target (MIR146A Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGS-CD64-2

    Plasmid

    #109191

    Purpose

    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    Full-length engineered CD64, derived from human CD64
    

    Use

    Tags

    Twin-Strep

    Expression

    Insect

    Mutation

    engineered for efficient expression, see publicat…

    Promoter

    Polyhedrin

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LSB-hsa-miR-221-5p

    Plasmid

    #103366

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-221-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-221-5p target (MIR221 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLY152

    Plasmid

    #130950

    Purpose

    dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    PpspA-2G6 and Ptet

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LSB-hsa-miR-186-5p

    Plasmid

    #103289

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-186-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-186-5p target (MIR186 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LSB-hsa-miR-221-3p

    Plasmid

    #103365

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-221-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-221-3p target (MIR221 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LSB-hsa-miR-191-5p

    Plasmid

    #103300

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-191-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-191-5p target (MIR191 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LSB-hsa-miR-150-5p

    Plasmid

    #103257

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-150-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-150-5p target (MIR150 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSB-hsa-miR-143-3p

    Plasmid

    #103242

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-143-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-143-3p target (MIR143 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXs-LB2O

    Plasmid

    #188036

    Purpose

    Fusion of human MYC Transactivation Domain (TAD) MYC Box II (MBII) with human OCT4 protein expressed in pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

    Insert

    MYC TAD MBII region fused with OCT4 (POU5F1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge