Addgene

We narrowed to 2,333 results for: Efs

Showing: 2301 - 2333 of 2333 results

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pSMP-Ehmt1_4

Plasmid

#36338

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Ehmt1_1

Plasmid

#36335

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xHA-MKK7a1-EE

Plasmid

#47578

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK7a1-EE (Map2k7 Mouse)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Residues 73-420 of RefSeq sequence. Ser271 to Gl…

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAWp40

Plasmid

#157980

Purpose

pFUGW-EFSp-BFP-{SbfI}

Depositor

Alan Siu Lun Wong

Article

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSMP-Ehmt1_3

Plasmid

#36337

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
sgRNA Clec12a (CT6)

Plasmid

#236203

Purpose

lentiviral expression of Cas9 and encodes CT6 sgRNA for CLEC12A deletion

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

Clec12a (Clec12a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA Clec12a (CT44)

Plasmid

#236204

Purpose

lentiviral expression of Cas9 and encodes CT44 sgRNA for CLEC12A deletion

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

Clec12a (Clec12a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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sgRNA Clec12a (CT47)

Plasmid

#236205

Purpose

lentiviral expression of Cas9 and encodes CT47 sgRNA for CLEC12A deletion

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

Clec12a (Clec12a Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK4-EE

Plasmid

#47577

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK4-EE (MAP2K4 Human)

Use

Tags

3xHA and GST

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

G245S

Promoter

Available since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pX68

Plasmid

#46857

Purpose

OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 A138V (TP53 Mouse, Human)

Use

Tags

Expression

Mammalian

Mutation

p53 A138V

Promoter

Available since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SCN1B-COMP5AP-AviTag-9xHis

Plasmid

#157099

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SCN1B (SCN1B Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRa

Plasmid

#134990

Purpose

dCas9-mediated activation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_ANKRD1_sgRNA2

Plasmid

#186669

Purpose

Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO5-SgHottip-GFP-CRISPRi

Plasmid

#134989

Purpose

dCas9-mediated inactivation of HOTTIP in mammalian cells

Depositor

Suming Huang

Article

Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

Insert

HOTTIP (HOTTIP Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

Plasmid

#162278

Purpose

Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

Depositor

Al George

Article

DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

Insert

SCN1A Variant 1, stabilized (SCN1A Human)

Use

Tags

IRES-mScarlet

Expression

Mammalian

Mutation

IVS intron inserted after bp2946. Silent codon c…

Promoter

CMV

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SCN1B-Fc(DAPA)-AviTag-6xHis

Plasmid

#156534

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SCN1B (SCN1B Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Nav1.7 Na+ channel scFv [N68/6]

Plasmid

#182094

Purpose

Mammalian Expression of Nav1.7 Na+ channel scFv. Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Nav1.7 Na+ channel (Homo sapiens) (SCN9A Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Nav1.7 Na+ channel [N68/6R]

Plasmid

#177566

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN9A Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A)R, gamma2 scFv [N452/81]

Plasmid

#182088

Purpose

Mammalian Expression of GABA(A)R, gamma2 scFv. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GABA(A)R, gamma2 (Homo sapiens) (GABRG2 Mouse)

Use

Tags

Sortase, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Nav1.7 Na+ channel [N68/6R-1]

Plasmid

#206717

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (SCN9A Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Nav1.7 Na+ channel [N68/6R-2b]

Plasmid

#206674

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant Mouse monoclonal antibody (SCN9A Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A)R, Gamma2L/S scFv [N452/30]

Plasmid

#206785

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/30 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A)R, Gamma2L/S scFv [N452/73]

Plasmid

#190555

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A)R, Gamma2L/S scFv [N452/81]

Plasmid

#190556

Purpose

Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GABA-A-R-Gamma2L [N452/69-2b] - Chimeric

Plasmid

#227036

Purpose

Mammalian expression plasmid of anti-GABA-A-R-Gamma2L (Human). Derived from hybridoma N452/69.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GABA-A-R-Gamma2L (Homo sapiens) recombinant mouse monoclonal antibody. (GABRG2 Mouse)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GABA(A)R, Gamma2L [N452/81R]

Plasmid

#177554

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L (Human). Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GABA(A)R, Gamma2L/S [N452/73R]

Plasmid

#177553

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L/S (Human). Derived from hybridoma N452/73.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L/S (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GABA(A)R, Gamma2L [N452/69]

Plasmid

#190331

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L (Human). Derived from hybridoma N452/69.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L (Homo sapiens) recombinant Mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-GABRG2 [N452/30]

Plasmid

#190330

Purpose

Mammalian Expression Plasmid of anti-GABRG2 (Human). Derived from hybridoma N452/30.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABRG2 (Homo sapiens) recombinant Mouse monoclonal antibody (GABRG2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge