161,407 results

1. 3xERRE/ERE-luciferase

   Plasmid

   #37852

   Depositor

   Rebecca Riggins

   Article

   Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

   Insert

   3 copies of ERRE/ERE upstream of luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

   Plasmid

   #175544

   Purpose

   Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag9-myc_PDGFRtmb
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRIPZ-bioGEF-UBnc-PURO

   Plasmid

   #208044

   Purpose

   Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   UBnc (UBC Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   L73P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Kir2.1-T2A-tdTomato

   Plasmid

   #60598

   Purpose

   Expresses Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 VDAC1-Linker-GFP

   Plasmid

   #211734

   Purpose

   Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

   Depositor

   Roland Malli

   Article

   Pilic et al bioRxiv 2023.09.08.556841

   Insert

   Voltage-dependent anion channel 1 (VDAC1 )
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJC2-Blast-SHMT2-3xFLAG

   Plasmid

   #233891

   Purpose

   lentiviral overexpression vector for SHMT2

   Depositor

   Jason Cantor

   Article

   Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

   Insert

   SHMT2 (SHMT2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. MP9956:SFG.GD3synthase-2A-GD2synthase

   Plasmid

   #75013

   Purpose

   Retroviral transfer vector which encodes the Disialoganglioside (GD2) mini-operon. Mammalian cells transduced with this vector become GD2 positive.

   Depositor

   Martin Pule

   Article

   Thomas et al PLoS One. 2016 Mar 31;11(3):e0152196. do

   Inserts

   murine GD3 synthase
   murine GD2 synthase
   

   Use

   Retroviral; Momlv retroviral vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE-BI-hGRAD

   Plasmid

   #207837

   Purpose

   Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   mCherry
   hFBXW11 (FBXW11 Human)
   VHH-GFP4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   215 amino acids of the full length protein

   Promoter

   TRE3G BI promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-loxp-DsRed-loxp-eGFP

    Plasmid

    #141148

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    loxp-DsRed-loxp-eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8.91

    Plasmid

    #187441

    Purpose

    Lentiviral packaging plasmid expressing gag and pol.

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Inserts

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAP05

    Plasmid

    #102920

    Purpose

    Direct cloning and broad-host-range heterologous expression vector

    Depositor

    Bradley Moore

    Article

    Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10875

    Plasmid

    #183963

    Purpose

    CBA-hyperdCas12a-HA-KRAB-mCherry

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, KRAB, and mCherry

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CBA

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS_12x-601_MA1

    Plasmid

    #114358

    Purpose

    12x Widom 601 nucleosome sequence

    Depositor

    Scot Wolfe

    Article

    Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

    Insert

    12X601-MA1
    

    Use

    Unspecified

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiPGK Blast DEST JNKKTRmRuby2

    Plasmid

    #59154

    Purpose

    Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFLP3

    Plasmid

    #64946

    Purpose

    Flp recombinase expression vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    Flp recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lambda promoter/temperature-sensitive Lambda cI r…

    Available since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIRT3 Flag

    Plasmid

    #13814

    Purpose

    Mammalian expression of human SIRT3 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT3 (SIRT3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. [#GS16-LV] Syn1-MitoTRACER - Donor

    Plasmid

    #233565

    Purpose

    Lentiviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells under the Synapsin1 promotor

    Depositor

    Simon Grelet

    Article

    Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

    Insert

    MitoTRACER-Donor
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    GFP11 and HA Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EGFP-N-Ezrin

    Plasmid

    #202684

    Purpose

    fluorescent protein tagged ezrin

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    Mus musculus EZR(1-586):EGFP (Ezr Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTH100

    Plasmid

    #84458

    Purpose

    pJB38-NWMN29-30 + SarA_P1-sGFP-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-sGFP from pCM29
    

    Use

    Tags

    6xHis (on sfGFP)

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-EZH2

    Plasmid

    #116738

    Purpose

    Lentiviral expression of EZH2

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-UBR5

    Plasmid

    #52050

    Purpose

    CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 (UBR5 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLentiCMV Puro DEST ERKKTRClover

    Plasmid

    #59150

    Purpose

    Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-alphasynuclein-WT

    Plasmid

    #40822

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pN1-PinkyCaMP

    Plasmid

    #232861

    Purpose

    A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRX-IP TFEB-sfGFP

    Plasmid

    #135402

    Purpose

    Expresses TFEB-sfGFP

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    TFEB (TFEB Human)
    

    Use

    Retroviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG

    Plasmid

    #176285

    Purpose

    Cre-dependent AAV virus

    Depositor

    The Allen Institute for Brain Science, Shenqin Yao

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    TVA66T-dTomato-rabies Glycoprotein
    

    Use

    Tags

    dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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