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pMSCV-Zeo

Plasmid

#75088

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Publication

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Publication

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Expression

Mammalian

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPSU10-30-50-100

Plasmid

#173838

Purpose

Coexpresses 10, 30, 50 and 100 kD Penn State ladder proteins

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPAB

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113341

Purpose

Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

VP64-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Expression

Mammalian

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Str-Golgin84_VSVG-SBP-EGFP

Plasmid

#65305

Purpose

synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pL-CRISPR.SFFV.GFP

Plasmid

#57827

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pCMV-Bx

Plasmid

#51552

Purpose

Expresses Bxb1 integrase in mammalian cells.

Depositor

Michele Calos

Publication

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

Bxb1 integrase

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCIN BRCA2 WT

Plasmid

#16245

Depositor

Mien-Chie Hung

Publication

Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX iLOV

Plasmid

#26587

Depositor

John Christie

Publication

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Tags

GST

Expression

Bacterial

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti6-DEST PINK1-V5 KD

Plasmid

#13319

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Kinase dead: K219A/D362A/D384A

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJL-SaKKH-BE3

Plasmid

#85170

Purpose

Expresses SaKKH-BE3 in mammalian cells [Edit]

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

SaKKH-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-Klf4-IP

Plasmid

#15920

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Kruppel-like factor 4 (gut) (Klf4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLKO.1-TSC2

Plasmid

#15478

Depositor

Do-Hyung Kim

Publication

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

TSC2 shRNA (TSC2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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PIK3CA - Exon20 H1047R

Plasmid

#16639

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Insert

PIK3CA H1047R (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

H1047R

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Publication

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Promoter

tetON

Available since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pHAGE-E-cadherin-RFP

Plasmid

#79603

Purpose

pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

Depositor

Jeffrey Rosen

Publication

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

CDH1 promoter region (CDH1 Human)

Use

Lentiviral

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Publication

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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